皮质类固醇对商陆丝裂原刺激的人淋巴细胞免疫球蛋白合成的增强作用。
Corticosteroid enhancement of immunoglobulin synthesis by pokeweed mitogen-stimulated human lymphocytes.
作者信息
Cooper D A, Duckett M, Petts V, Penny R
出版信息
Clin Exp Immunol. 1979 Jul;37(1):145-51.
The effects of the addition in vitro of corticosteroid on pokeweed mitogen (PWM) induced Ig synthesis by human peripheral blood lymphocytes were studied. IgG in supernatants produced under standardized culture conditions was measured by double antibody radioimmunoassay. The addition of 10(-6)M prednisolone caused a remarkable enhancement of PWM-stimulated IgG synthesis beginning at day 4 of culture and increasing at a faster rate than that in cultures with PWM alone. 10(-6)M prednisolone resulted in a geometric mean enhancement of 5.6-fold of PWM-stimulated IgG synthesis in all twenty-five normal controls studied. This enhancement occurred up to 3 days after the addition of PWM. 10(-6)M and 10(-5)M prednisolone resulted in significantly greater enhancement of PWM-stimulated IgG synthesis than 10(-7)M prednisolone. Hydrocortisone, prednisolone, methylprednisolone, betamethasone and dexamethasone at 10(-6)M were all equally effective in the enhancement of PWM-induced IgG synthesis.
研究了体外添加皮质类固醇对人外周血淋巴细胞受商陆丝裂原（PWM）诱导的Ig合成的影响。通过双抗体放射免疫测定法测量在标准化培养条件下产生的上清液中的IgG。添加10⁻⁶M泼尼松龙后，从培养第4天开始，PWM刺激的IgG合成显著增强，且其增长速度比仅用PWM培养的更快。在所研究的25名正常对照中，10⁻⁶M泼尼松龙使PWM刺激的IgG合成几何平均增强了5.6倍。这种增强在添加PWM后持续3天。10⁻⁶M和10⁻⁵M泼尼松龙比10⁻⁷M泼尼松龙能更显著地增强PWM刺激的IgG合成。10⁻⁶M的氢化可的松、泼尼松龙、甲泼尼龙、倍他米松和地塞米松在增强PWM诱导的IgG合成方面同样有效。
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