Januszewicz P, Thibault G, Garcia R, Gutkowska J, Genest J, Cantin M
Biochem Biophys Res Commun. 1986 Jan 29;134(2):652-8. doi: 10.1016/s0006-291x(86)80469-7.
The effect of synthetic rat atrial natriuretic factor (ANF, Arg 101-Tyr 126) was evaluated in an in-vitro model of rat hypothalamo-neurohypophysial complex (HNC) in organ culture in which part of hypothalamus containing a portion of undamaged magnocellular neurons is separated from posterior pituitary by a fluid tight barrier with an intact stalk connecting both structures. ANF, when added to the medium at the hypothalamus site at concentrations of 3 X 10(-5) M to 3 X 10(-7) M, did not change basal AVP release from the posterior pituitary. Similarly, a shorter form of ANF (Cys 105-Tyr 126), reported to be highly potent in inhibiting adenylate cyclase activity in various tissues, exerted no effect on AVP excretion from HNC in organ culture. The application of an hyperosomotic medium (osmolality 324 +/- 2 mOsm/kg H2O) to the hypothalamic side, together with ANF (3 X 10(-6) M), significantly lowered osmotically-stimulated AVP release. It is concluded that ANF has no effect on basal AVP release from HNC in culture and suppresses osmotically-stimulated AVP secretion in this in vitro model.
在大鼠下丘脑 - 神经垂体复合体（HNC）器官培养的体外模型中评估了合成大鼠心房利钠因子（ANF，精氨酸101 - 酪氨酸126）的作用。在该模型中，含有一部分未受损大细胞神经元的下丘脑部分通过液密屏障与垂体后叶分离，且有完整的柄连接这两个结构。当以3×10⁻⁵ M至3×10⁻⁷ M的浓度在下丘脑部位将ANF添加到培养基中时，垂体后叶的基础抗利尿激素（AVP）释放没有变化。同样，据报道在抑制各种组织中的腺苷酸环化酶活性方面具有高效力的较短形式的ANF（半胱氨酸105 - 酪氨酸126），对器官培养中的HNC的AVP排泄没有影响。将高渗培养基（渗透压324±2 mOsm/kg H₂O）应用于下丘脑一侧，并加入ANF（3×10⁻⁶ M），可显著降低渗透压刺激的AVP释放。得出的结论是，在培养中ANF对HNC的基础AVP释放没有影响，并且在这个体外模型中抑制渗透压刺激的AVP分泌。
