2型糖尿病患者尿β-微球蛋白基因表达分析
Urinary beta-trace protein gene expression analysis in type 2 diabetes mellitus patients.
作者信息
Bacci Marcelo Rodrigues, Alves Beatriz da Costa Aguiar, Cavallari Marina Romera, Azzalis Ligia Ajaime, Rozier-Alves Ross Martin de, Perez Matheus Moreira, Chehter Ethel Zimberg, Pereira Edimar Cristiano, Fonseca Fernando Luiz Affonso
机构信息
Faculdade de Medicina do ABC, Santo André, SP, Brazil.
Universidade Federal de São Paulo, Diadema, SP, Brazil.
出版信息
Einstein (Sao Paulo). 2017 Oct-Dec;15(4):441-444. doi: 10.1590/S1679-45082017AO4012.
OBJECTIVE
To evaluate the gene expression of beta-trace protein in urine of diabetic patients, with no reduction in glomerular filtration rate, which was defined as below 60mL/min/1.73m2.
METHODS
Type 2 diabetes mellitus patients were recruited, and a group of non-diabetic individuals served as control. Beta-trace protein gene expression was analyzed by quantitative PCR. Blood samples were collected to establish glucose levels and baseline kidney function. Accuracy was analyzed using ROC curves.
RESULTS
Ninety type 2 diabetes mellitus patients and 20 non-diabetic individuals were recruited. The area under the curve was 0.601, sensitivity of 20% and specificity of 89.47%. Among diabetic participants, 18% showed an expression above the cutoff point.
CONCLUSION
These results of accuracy of beta-trace protein gene expression in urine of diabetic patients are promising, although they did not achieve a higher area under the curve level.
目的
评估肾小球滤过率未降低（定义为低于60mL/min/1.73m²）的糖尿病患者尿液中β-微球蛋白的基因表达。
方法
招募2型糖尿病患者，并选取一组非糖尿病个体作为对照。通过定量PCR分析β-微球蛋白基因表达。采集血样以确定血糖水平和基线肾功能。使用ROC曲线分析准确性。
结果
招募了90名2型糖尿病患者和20名非糖尿病个体。曲线下面积为0.601，敏感性为20%，特异性为89.47%。在糖尿病参与者中，18%的人表达高于临界值。
结论
糖尿病患者尿液中β-微球蛋白基因表达准确性的这些结果很有前景，尽管它们未达到更高的曲线下面积水平。
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