血管生成素通过减少成纤维细胞增殖和转化生长因子β1分泌来减轻烧伤患者的瘢痕形成。
Angiogenin Attenuates Scar Formation in Burn Patients by Reducing Fibroblast Proliferation and Transforming Growth Factor β1 Secretion.
作者信息
Pan Shin-Chen, Lee Chou-Hwei, Chen Chung-Lin, Fang Wei-Yu, Wu Li-Wha
出版信息
Ann Plast Surg. 2018 Feb;80(2S Suppl 1):S79-S83. doi: 10.1097/SAP.0000000000001306.
BACKGROUND
Deep burn wounds have a high tendency to form hypertrophic scars. Previously, we found that angiogenin promoted neovascularization during deep burn wound healing. However, the association between angiogenin and scar formation is unclear.
METHODS
We obtained human burn scar tissues from patients who underwent scar surgery and examined the role of angiogenin in scar tissues and determined its effects in scar fibroblasts and on transforming growth factor β1 (TGF-β1) secretion.
RESULTS
Our results showed an inverse correlation between angiogenin expression and scar severity. Next, we examined the effects of angiogenin in scar fibroblasts. We found that angiogenin was persistently expressed in human scar fibroblasts and that angiogenin expression significantly increased with time in the culture medium of scar fibroblasts. Treatment of scar fibroblasts with recombinant angiogenin significantly decreased their proliferation and TGF-β1 secretion. Moreover, angiogenin inhibited TGF-β1-mediated Smad2 signaling pathway.
CONCLUSION
Our data suggest a negative role of angiogenin in fibroblast proliferation via TGF-β1-mediated Smad2 signaling pathway.
背景
深度烧伤创面极易形成增生性瘢痕。此前，我们发现血管生成素在深度烧伤创面愈合过程中促进新生血管形成。然而，血管生成素与瘢痕形成之间的关联尚不清楚。
方法
我们从接受瘢痕手术的患者身上获取人烧伤瘢痕组织，研究血管生成素在瘢痕组织中的作用，并确定其对瘢痕成纤维细胞及转化生长因子β1（TGF-β1）分泌的影响。
结果
我们的结果显示血管生成素表达与瘢痕严重程度呈负相关。接下来，我们研究了血管生成素对瘢痕成纤维细胞的影响。我们发现血管生成素在人瘢痕成纤维细胞中持续表达，且在瘢痕成纤维细胞培养基中，血管生成素表达随时间显著增加。用重组血管生成素处理瘢痕成纤维细胞可显著降低其增殖及TGF-β1分泌。此外，血管生成素抑制TGF-β1介导的Smad2信号通路。
结论
我们的数据表明血管生成素通过TGF-β1介导的Smad2信号通路对成纤维细胞增殖起负向作用。
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