Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China.
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China.
Molecules. 2018 Jan 25;23(2):239. doi: 10.3390/molecules23020239.
Polysaccharides are closely associated with immune regulation, but there are different polysaccharide effects from different sources. In this study, the aim was to investigate the effect of tremella polysaccharides (TP) in cyclophosphamide-induced immunodeficient mice. We observed the thymus and spleen index, liver and spleen pathological changes, and the levels of IL-2, IL-12, INF-γ, TGF-β and Ig G in serum, and we also noted the mRNA expression of IL-1β, IL-4, IL-12 and TGF-β in liver and spleen. Besides, we also measured the best effects of different doses of TP (Low-TP was 20 mg/kg·BW, Middle-TP was 40 mg/kg·BW, and High-TP was 80 mg/kg·BW) on cyclophosphamide-induced immunosuppressed mice. The results were remarkable, and suggested that TP had a significant effect for enhancing immunity in cyclophosphamide-induced immunosuppression, and the immune enhancement of High-TP had the best results in TP-treated mice. It could significantly increase the thymus and spleen index, alleviate pathological features of immunosuppression such as the arrangement of liver sinusoid and hepatic plates was disordered, massive inflammatory cells infiltrated and fatty degeneration of hepatocytes in liver, and red pulp and white pulp were intermixed, splenic corpuscles demolished and disappeared, splenic sinusoid extended, and lymphocytes of spleen were reduced in spleen. Besides, it could also up-regulate serum levels of IL-2, IL-12, INF-γ and Ig G, reduce the level of TGF-β in serum, markedly promote mRNA expression of IL-1β, IL-4 and IL-12 in liver and spleen, and suppress mRNA expression of TGF-β. Above all, TP showed preventive effect for cyclophosphamide-induced immunosuppressed mice.
多糖与免疫调节密切相关，但不同来源的多糖具有不同的作用。本研究旨在探讨银耳多糖（TP）对环磷酰胺致免疫低下小鼠的作用。观察了胸腺和脾脏指数、肝脾组织病理学变化以及血清中 IL-2、IL-12、INF-γ、TGF-β和 IgG 水平，并检测了肝脾组织中 IL-1β、IL-4、IL-12 和 TGF-β 的 mRNA 表达。此外，还测定了不同剂量 TP（低剂量 TP 为 20mg/kg·BW，中剂量 TP 为 40mg/kg·BW，高剂量 TP 为 80mg/kg·BW）对环磷酰胺致免疫抑制小鼠的最佳作用。结果表明，TP 对环磷酰胺致免疫抑制有显著的增强免疫作用，高剂量 TP 的免疫增强作用在 TP 处理的小鼠中效果最佳。它能显著增加胸腺和脾脏指数，减轻肝窦和肝板排列紊乱、大量炎症细胞浸润和肝细胞脂肪变性、红髓和白髓混合、脾小体破坏和消失、脾窦扩张、脾淋巴细胞减少等免疫抑制的病理特征。此外，它还能上调血清中 IL-2、IL-12、INF-γ和 IgG 的水平，降低 TGF-β 的水平，显著促进肝脾组织中 IL-1β、IL-4 和 IL-12 的 mRNA 表达，抑制 TGF-β 的 mRNA 表达。综上所述，TP 对环磷酰胺致免疫低下小鼠有预防作用。
