Divisions of Biostatistics and Epidemiology, School of Public Health, University of California , Berkeley, California 94720, United States.
Section of Cell and Developmental Biology, University of California, San Diego , La Jolla, California 92093, United States of America.
ACS Chem Biol. 2018 Feb 16;13(2):424-430. doi: 10.1021/acschembio.7b00923. Epub 2018 Feb 7.
The recent discovery of CRISPR and its application as a gene editing tool has enabled a range of gene drive systems to be engineered with greater ease. In order for the benefits of this technology to be realized, in some circumstances drive systems should be developed that are capable of both spreading into populations to achieve their desired impact and being recalled in the event of unwanted consequences or public disfavor. We review the performance of three broad categories of drive systems at achieving these goals: threshold-dependent drives, homing-based drive and remediation systems, and temporally self-limiting systems such as daisy-chain drives.
最近发现的 CRISPR 及其作为基因编辑工具的应用使得一系列基因驱动系统的设计变得更加容易。为了实现这项技术的好处，在某些情况下，应该开发出能够传播到种群中以实现预期效果、并在出现意外后果或公众不满时被召回的驱动系统。我们回顾了三种广泛的驱动系统在实现这些目标方面的性能：依赖阈值的驱动、基于同源的驱动和修复系统，以及临时自我限制的系统，如雏菊链驱动。
