Repasky E A, Eckert B S
Cell Tissue Res. 1981;221(1):85-91. doi: 10.1007/BF00216572.
The process of nuclear extrusion continues when erythroid cells of mouse spleen are placed in vitro, although the process may be considerably retarded. This allows visualization of unusually large numbers of enucleating cells after 30 min in vitro. With this system, the mechanism of enucleation can be analyzed both qualitatively and quantitatively. We have used this system to study the effects of cytochalasin B and have found it to be a potent inhibitor of enucleation. These results suggest that microfilaments are involved in the process of enucleation.
当将小鼠脾脏的红细胞置于体外时，核挤出过程仍会继续，尽管该过程可能会显著延迟。这使得在体外培养30分钟后能够观察到大量异常的去核细胞。利用这个系统，可以从定性和定量两个方面分析去核机制。我们已使用该系统研究细胞松弛素B的作用，发现它是去核的有效抑制剂。这些结果表明微丝参与了去核过程。
