Wagmann Lea, Maurer Hans H
Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg, Germany.
Handb Exp Pharmacol. 2018;252:413-439. doi: 10.1007/164_2017_83.
Bioanalysis of new psychoactive substances (NPS) is very challenging due to the growing number of compounds with new chemical structures found on the drugs of abuse market. Screening, identification, and quantification in biosamples are needed in clinical and forensic toxicology settings, and these procedures are more challenging than the analysis of seized drug material because of extremely low concentrations encountered in biofluids but also due to diverse metabolic alterations of the parent compounds. This article focuses on bioanalytical single- and multi-analyte procedures applicable to a broad variety of NPS in various biomatrices, such as blood, urine, oral fluid, or hair. Sample preparation, instrumentation, detection modes, and data evaluation are discussed as well as corresponding pitfalls. PubMed-listed and English-written original research papers and review articles published online between 01 October 2012 and 30 September 2017 were considered.
由于在滥用药物市场上发现的具有新化学结构的化合物数量不断增加，新型精神活性物质（NPS）的生物分析极具挑战性。在临床和法医毒理学环境中，需要对生物样本进行筛查、鉴定和定量，这些程序比分析查获的毒品材料更具挑战性，这是因为生物流体中浓度极低，而且母体化合物还存在多种代谢变化。本文重点介绍适用于各种生物基质（如血液、尿液、口腔液或毛发）中多种NPS的生物分析单分析物和多分析物程序。讨论了样品制备、仪器、检测模式和数据评估以及相应的陷阱。考虑了2012年10月1日至2017年9月30日期间在线发表的PubMed列出的英文原创研究论文和综述文章。
