Mandarano Alexandra H, Giloteaux Ludovic, Keller Betsy A, Levine Susan M, Hanson Maureen R
Department of Molecular Biology & Genetics, Cornell University, Ithaca, NY, United States of America.
Department of Exercise & Sport Sciences, Ithaca College, Ithaca, NY, United States of America.
PeerJ. 2018 Jan 22;6:e4282. doi: 10.7717/peerj.4282. eCollection 2018.
Patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) often suffer from gastrointestinal symptoms and many are diagnosed with irritable bowel syndrome (IBS). Previous studies, including from our laboratory, have demonstrated that the ME/CFS gut bacterial composition is altered and less diverse when compared to healthy individuals. Patients have increased biomarkers of inflammation and leaky gut syndrome. To further investigate dysbiosis in the ME/CFS gut microbiome, we sought to characterize the eukaryotes present in the gut of 49 individuals with ME/CFS and 39 healthy controls. Using 18S rRNA sequencing, we have identified eukaryotes in stool samples of 17 healthy individuals and 17 ME/CFS patients. Our analysis demonstrates a small, nonsignificant decrease in eukaryotic diversity in ME/CFS patients compared to healthy individuals. In addition, ME/CFS patients show a nonsignificant increase in the ratio of fungal phyla to , which is consistent with ongoing inflammation in ME/CFS. We did not identify specific eukaryotic taxa that are associated with ME/CFS disease status.
肌痛性脑脊髓炎/慢性疲劳综合征（ME/CFS）患者常伴有胃肠道症状，许多患者被诊断为肠易激综合征（IBS）。包括我们实验室在内的先前研究表明，与健康个体相比，ME/CFS患者的肠道细菌组成发生改变且多样性降低。患者的炎症生物标志物和肠漏综合征增加。为了进一步研究ME/CFS肠道微生物群的失调情况，我们试图对49名ME/CFS患者和39名健康对照者肠道中的真核生物进行特征描述。使用18S rRNA测序，我们在17名健康个体和17名ME/CFS患者的粪便样本中鉴定出了真核生物。我们的分析表明，与健康个体相比，ME/CFS患者的真核生物多样性有小幅但不显著的降低。此外，ME/CFS患者中真菌门与的比例有不显著的增加，这与ME/CFS中持续的炎症一致。我们没有发现与ME/CFS疾病状态相关的特定真核生物分类群。
