Rainer Georg, Kuhnert Regina, Unterholzer Mara, Dresch Philipp, Gruber Andreas, Peintner Ursula
Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, A-6020 Innsbruck, Austria.
Institute of Botany, University of Innsbruck, Sternwartestraße 15, A-6020 Innsbruck, Austria.
J Fungi (Basel). 2015 Apr 30;1(1):55-75. doi: 10.3390/jof1010055.
Ectomycorrhizae (EM) are important for the survival of seedlings and trees, but how they will react to global warming or changes in soil fertility is still in question. We tested the effect of soil temperature manipulation and nitrogen fertilization on EM communities in a high-altitude afforestation. The trees had been inoculated in the 1960s in a nursery with a mixture of , and Sampling was performed during the third year of temperature manipulation in June and October 2013. Root tips were counted, sorted into morphotypes, and sequenced. Fungal biomass was measured as ergosterol and hyphal length. The EM potential of the soil was assessed with internal transcribed spacers (ITS) clone libraries from in-growth mesh bags (MB). Temperature manipulation of ± 1 °C had no effect on the EM community. A total of 33 operational taxonomic units (OTUs) were identified, 20 from the roots, 13 from MB. The inoculated spp. colonized 82% of the root tips, thus demonstrating that the inoculation was sustainable. Nitrogen fertilization had no impact on the EM community, but promoted depletion in soil organic matter, and caused a reduction in soil fungal biomass.
外生菌根（EM）对幼苗和树木的存活至关重要，但它们如何应对全球变暖和土壤肥力变化仍存在疑问。我们在高海拔造林中测试了土壤温度控制和氮肥施用对EM群落的影响。这些树木于20世纪60年代在苗圃中接种了 、 和 的混合物。在2013年6月和10月温度控制的第三年进行采样。对根尖进行计数，分类为形态型并测序。真菌生物量以麦角固醇和菌丝长度来衡量。利用来自内生长网袋（MB）的内部转录间隔区（ITS）克隆文库评估土壤的EM潜力。±1°C的温度控制对EM群落没有影响。共鉴定出33个操作分类单元（OTU），其中20个来自根系，13个来自MB。接种的 属定殖在82%的根尖上，从而表明接种是可持续的。氮肥对EM群落没有影响，但促进了土壤有机质的消耗，并导致土壤真菌生物量减少。
