Department of Biological Materials, CSIR - Central Leather Research Institute, Adyar, Chennai 600020, India.
Centre for Nanobiotechnology (CNBT), VIT, Vellore 632014, India.
Int J Biol Macromol. 2018 Jun;112:250-257. doi: 10.1016/j.ijbiomac.2018.01.159. Epub 2018 Feb 3.
Bionanocomposites fabricated using metal nanoparticles serve a wide range of biomedical applications viz., site targeted drug delivery, imaging etc. Theranostics emerge as an important field of science, which focuses on the use of single entity for both disease diagnosis and treatment. The present work aimed at designing a multifunctional nanocomposite comprising of iron/gold hybrid nanoparticles, coated with oleyl chitosan and conjugated with methotrexate. The HR-TEM images revealed the spherical nature of the composite, while it's nontoxic and biocompatible property was proved by the MTT assay in NIH 3T3 cells and hemolysis assay. Though the VSM results exhibited the magnetic property, the MRI phantom images and X-ray contrast images demonstrated the potential of the composite to be used as contrast agent. Thus the prepared nanocomposite possess good cytocompatibility, magnetic property and also high X-ray attenuation, wherein it could serve as a novel platform for both MRI and CT diagnosis, as well as drug conjugation could aid in targeted drug delivery.
使用金属纳米粒子制造的生物纳米复合材料在广泛的生物医学应用中发挥作用，例如靶向药物输送、成像等。治疗诊断学是一个重要的科学领域，专注于使用单一实体进行疾病诊断和治疗。本工作旨在设计一种多功能纳米复合材料，该复合材料由铁/金杂化纳米粒子组成，用油酰壳聚糖包覆，并与甲氨蝶呤偶联。高分辨率透射电子显微镜（HR-TEM）图像显示了该复合材料的球形性质，而其在 NIH 3T3 细胞和溶血试验中的 MTT 测定证明了其无毒和生物相容性。虽然振动样品磁强计（VSM）结果显示出了磁性，但磁共振成像（MRI）和 X 射线造影图像显示了该复合材料作为造影剂的潜力。因此，所制备的纳米复合材料具有良好的细胞相容性、磁性和高 X 射线衰减率，可作为 MRI 和 CT 诊断的新型平台，并且药物偶联可以辅助靶向药物输送。
