Farjadian Fatemeh, Moradi Sahar, Hosseini Majid
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH, 44325, USA.
J Mater Sci Mater Med. 2017 Mar;28(3):47. doi: 10.1007/s10856-017-5854-2. Epub 2017 Feb 7.
Magnetic nanoparticles have found application as MRI contrasting agents. Herein, chitosan thin films containing super-paramagnetic iron oxide nanoparticles (SPIONs) are evaluated in magnetic resonance imaging (MRI). To determine their contrasting capability, super-paramagnetic nanoparticles coated with citrate (SPIONs-cit) were synthesized. Then, chitosan thin films with different concentrations of SPIONs-cit were prepared and their MRI data (i.e., r and r *) was evaluated in an aqueous medium. The synthesized SPIONs-cit and chitosan/SPIONs-cit films were characterized by FTIR, EDX, XRD as well as VSM with the morphology evaluated by SEM and AFM. The nanoparticle sizes and distribution confirmed well-defined nanoparticles and thin films formation along with high contrasting capability in MRI. Images revealed well-dispersed uniform nanoparticles, averaging 10 nm in size. SPIONs-cit's hydrodynamic size averaged 23 nm in diameter. The crystallinity obeyed a chitosan and SPIONs pattern. The in vitro cellular assay of thin films with a novel route was performed within Hek293 cell lines showing that thin films can be biocompatible.
磁性纳米颗粒已被用作磁共振成像（MRI）造影剂。在此，对含有超顺磁性氧化铁纳米颗粒（SPIONs）的壳聚糖薄膜进行磁共振成像（MRI）评估。为了确定它们的造影能力，合成了包覆柠檬酸盐的超顺磁性纳米颗粒（SPIONs-cit）。然后，制备了含有不同浓度SPIONs-cit的壳聚糖薄膜，并在水介质中评估了它们的MRI数据（即r和r*）。通过傅里叶变换红外光谱（FTIR）、能量色散X射线光谱（EDX）、X射线衍射（XRD）以及振动样品磁强计（VSM）对合成的SPIONs-cit和壳聚糖/SPIONs-cit薄膜进行了表征，通过扫描电子显微镜（SEM）和原子力显微镜（AFM）评估了其形态。纳米颗粒的尺寸和分布证实形成了明确的纳米颗粒和薄膜，并且在MRI中具有高造影能力。图像显示纳米颗粒分散均匀，平均尺寸为10纳米。SPIONs-cit的流体动力学直径平均为23纳米。结晶度符合壳聚糖和SPIONs的模式。在Hek293细胞系中采用新方法对薄膜进行了体外细胞测定，结果表明薄膜具有生物相容性。
