Aleanizy Fadilah Sfouq, Alqahtani Fulwah Yahya, Shazly Gamal, Alfaraj Rihaf, Alsarra Ibrahim, Alshamsan Aws, Gareeb Abdulhady Hosam
Nanomedicine Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Saudi Pharm J. 2018 Jan;26(1):79-83. doi: 10.1016/j.jsps.2017.10.009. Epub 2017 Oct 14.
The purpose of this study was to study the antimicrobial activity of chitosan nanoparticles (CSNPs) on with special emphasis on their sensitivity to pH and the effect of pH on their activity.
Antimicrobial activity of CSNPs against at different pH was tested using broth dilution method. Further assessment of antivirulence activity and sensitization of CSNPs on were examined
Significant antimicrobial effects of CSNPs against were detected at slightly acidic pH 5, whereas the activity was abolished at a pH of greater than 7. The antivirulence activity of CSNPs was then investigated and treatment with CSNPs (1000 ppm) resulted in a significant reduction or even complete inhibition of pyocyanin production by compared with untreated indicating the antivirulence activity of CSNPs. CSNPs also sensitized to the lytic effects of sodium dodecyl sulfate (SDS); such sensitization was not blocked by washing chitosan-treated cells prior to SDS exposure revealing that CSNPs disturb the outer membrane leading to irreversible sensitivity to detergent even at low concentration (100 ppm).
These findings highlight CSNPs as potentially useful as indirect antimicrobial agents for a variety of applications.
本研究旨在研究壳聚糖纳米颗粒（CSNPs）的抗菌活性，特别强调其对pH的敏感性以及pH对其活性的影响。
采用肉汤稀释法测试CSNPs在不同pH值下对[具体受试对象未明确]的抗菌活性。进一步评估了CSNPs对[具体受试对象未明确]的抗毒力活性和致敏作用。
在微酸性pH 5时检测到CSNPs对[具体受试对象未明确]有显著的抗菌作用，而在pH大于7时活性消失。随后研究了CSNPs的抗毒力活性，与未处理的[具体受试对象未明确]相比，用CSNPs（1000 ppm）处理导致[具体受试对象未明确]产生的绿脓菌素显著减少甚至完全抑制，表明CSNPs具有抗毒力活性。CSNPs还使[具体受试对象未明确]对十二烷基硫酸钠（SDS）的裂解作用敏感；在SDS暴露前洗涤壳聚糖处理的细胞并不能阻止这种致敏作用，这表明CSNPs会干扰外膜，导致即使在低浓度（100 ppm）下对去污剂也具有不可逆的敏感性。
这些发现突出了CSNPs作为潜在有用的间接抗菌剂在多种应用中的可能性。
