Abusrewil Sumaya, Brown Jason L, Delaney Christopher, Butcher Mark C, Tiba Mohammed, Scott J Alun, Ramage Gordon, McLean William
Glasgow Endodontology Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, Glasgow G12 8QF, UK.
Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, 378 Sauchiehall Street, Glasgow G2 3JZ, UK.
Antibiotics (Basel). 2021 Oct 29;10(11):1317. doi: 10.3390/antibiotics10111317.
Endodontic infection is a biofilm disease that is difficult to irradicate with current treatment protocols, and as such, persistent micro-organisms may lead to ongoing or recurrent disease. The potential for the use of enhanced filling materials to modify biofilm regrowth is a promising strategy. This current study aimed to evaluate the anti-biofilm efficacy of calcium silicate cements modified with chitosan. The development of mono-species and multi-species biofilms on ProRoot MTA, Biodentine and bovine dentine discs were explored using quantitative microbiology analysis. The effect on regrowth of biofilms was assessed following the addition of chitosan to each cement. In comparison to a dentine substrate, both materials did not show the ability to inhibit biofilm regrowth. Biodentine incorporated with chitosan displayed a dose-dependent reduction in multi-species biofilm regrowth, unlike MTA. Notably, interkingdom biofilms were shown to enhance bacterial tolerance in the presence of chitosan. This study demonstrates the potential to enhance the antimicrobial properties of Biodentine. The findings highlight the need for appropriate model systems when exploring antimicrobial properties of materials in vitro so that interspecies and interkingdom interactions that modify tolerance are not overlooked while still supporting the development of innovative materials.
牙髓感染是一种生物膜疾病，目前的治疗方案难以根除，因此，持续存在的微生物可能导致疾病持续或复发。使用增强型填充材料来改变生物膜再生长的潜力是一种很有前景的策略。本研究旨在评估壳聚糖改性硅酸钙水门汀的抗生物膜效果。使用定量微生物学分析方法，探索了单菌种和多菌种生物膜在ProRoot MTA、BioDentine和牛牙本质圆盘上的形成情况。在每种水门汀中添加壳聚糖后，评估其对生物膜再生长的影响。与牙本质基质相比，这两种材料均未显示出抑制生物膜再生长的能力。与MTA不同，添加壳聚糖的BioDentine在多菌种生物膜再生长方面呈现出剂量依赖性降低。值得注意的是，在壳聚糖存在的情况下，跨界生物膜显示出增强细菌耐受性的作用。本研究证明了增强BioDentine抗菌性能的潜力。研究结果强调，在体外探索材料的抗菌性能时，需要合适的模型系统，以便在支持创新材料开发的同时，不忽视改变耐受性的种间和跨界相互作用。
