Yue Bei People's Hospital, Shaoguan 512026, China.
State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China.
J Diabetes Res. 2017;2017:3417306. doi: 10.1155/2017/3417306. Epub 2017 Dec 12.
Glucose and lipid metabolism disorder in diabetes mellitus often causes damage to multiple tissues and organs. Diabetes mellitus is beneficially affected by quercetin. However, its concrete mechanisms are yet to be fully elucidated. In our study, diabetes was induced in Sprague-Dawley rats by STZ injection. The rats were randomly divided into normal control, diabetic model, low-dose quercetin treatment, high-dose quercetin treatment, and pioglitazone treatment groups. Fasting blood glucose was collected to evaluate diabetes. Immunohistochemistry and fluorometric assay were performed to explore SIRT1. Akt levels were measured through immunoprecipitation and Western blot. After 12 weeks of quercetin treatment, the biochemical parameters of glucose and lipid metabolism improved to varying degrees. Hepatic histomorphological injury was alleviated, and hepatic glycogen content was increased. The expression and activity of hepatic SIRT1 were enhanced, and Akt was activated by phosphorylation and deacetylation. These results suggested that the beneficial effects of quercetin on glucose and lipid metabolism disorder are probably associated with the upregulated activity and protein level of SIRT1 and its influence on Akt signaling pathway. Hence, quercetin shows potential for the treatment of glucose and lipid metabolism disorder in diabetes mellitus.
糖尿病患者的糖脂代谢紊乱常导致多组织器官损伤。槲皮素对糖尿病有有益影响。然而，其具体机制尚未完全阐明。在我们的研究中，通过 STZ 注射诱导 Sprague-Dawley 大鼠发生糖尿病。大鼠随机分为正常对照组、糖尿病模型组、低剂量槲皮素治疗组、高剂量槲皮素治疗组和吡格列酮治疗组。采集空腹血糖以评估糖尿病。通过免疫组化和荧光测定法来探究 SIRT1。通过免疫沉淀和 Western blot 来测量 Akt 水平。经过 12 周的槲皮素治疗，糖脂代谢的生化参数得到不同程度的改善。肝组织形态损伤减轻，肝糖原含量增加。肝 SIRT1 的表达和活性增强，Akt 通过磷酸化和去乙酰化被激活。这些结果表明，槲皮素对糖脂代谢紊乱的有益作用可能与 SIRT1 活性和蛋白水平的上调及其对 Akt 信号通路的影响有关。因此，槲皮素显示出治疗糖尿病糖脂代谢紊乱的潜力。
