Suekawa Marina, Fujikawa Yukichi, Esaka Muneharu
a Graduate School of Biosphere Sciences , Hiroshima University , Higashi-Hiroshima , Japan.
Biosci Biotechnol Biochem. 2018 Mar;82(3):425-432. doi: 10.1080/09168451.2018.1429887. Epub 2018 Jan 31.
Aldo-keto reductases (AKRs) play important roles in aldehyde detoxification as well as primary and secondary metabolism in plants. We previously reported inducible expression of a Solanum lycopersicum AKR4B (SlAKR4B) in tomato leaves treated with salicylic acid and jasmonic acid, and high promoter activity of SlAKR4B in tomato leaf protoplasts. In this study, we investigated the expression response of SlAKR4B in the tomato leaves with infiltration treatment and the cis-element(s) involved in high promoter activity. Gene expression analysis in tomato leaf protoplasts and buffer-infiltrated tomato leaves suggested that cell damage caused the increased expression of SlAKR4B. Promoter activity of SlAKR4B was significantly reduced by mutation of two G-box like elements. It is suggested that the two G-box like elements are responsible for the high promoter activity.
醛酮还原酶（AKRs）在植物的醛解毒以及初级和次级代谢中发挥着重要作用。我们之前报道了水杨酸和茉莉酸处理的番茄叶片中番茄醛酮还原酶4B（SlAKR4B）的诱导表达，以及SlAKR4B在番茄叶原生质体中的高启动子活性。在本研究中，我们研究了SlAKR4B在浸润处理的番茄叶片中的表达反应以及参与高启动子活性的顺式元件。番茄叶原生质体和缓冲液浸润的番茄叶片中的基因表达分析表明，细胞损伤导致SlAKR4B表达增加。两个类G-box元件的突变显著降低了SlAKR4B的启动子活性。表明这两个类G-box元件负责高启动子活性。
