College of Food and Biological Engineering, Qiqihar University, Qiqihar, 161006, China.
College of Life Sciences, Agriculture and Forestry, Qiqihar University, Qiqihar, 161006, China.
Sci Rep. 2022 Jun 30;12(1):11098. doi: 10.1038/s41598-022-15451-9.
Chlorophyll loss is a major problem during green vegetable storage. However, the molecular mechanism is still unclear. In this study, a 21 days of storage experiments showed chlorophyll content was higher in light-stored Chinese cabbage (Brassica chinensis L.) leaves than those in dark-stored samples. Transcriptome analyses were performed on these samples to determine the effects of light. Among 311 differentially expressed genes (DEGs), early light-induced protein 1 (ELIP1) was identified as the main control gene for chlorophyll synthesis. Tissues and subcellular localization indicated that ELIP1 was localized in the nucleus. Motifs structure analyses, chromatin immunoprecipitation (ChIP) assays, luciferase reporter assays, and overexpression experiments demonstrated that ELIP1 regulated the expressions of genomes uncoupled 4 (GUN4), Glutamyl-tRNA reductase family protein (HEMA1), and Mg-protoporphyrin IX methyltransferase (CHLM) by binding to G-box-like motifs and affected chlorophyll biosynthesis during the storage of Chinese cabbage. It is a possible common tetrapyrrole biosynthetic pathway for chlorophylls, hemes, and bilin pigments in photosynthetic organisms. Our research also revealed that white light can be used as a regulatory factor to improve the storage ability and extent shelf life of Chinese cabbage.
叶绿素的损失是绿叶蔬菜储存过程中的一个主要问题。然而，其分子机制尚不清楚。在这项研究中，经过 21 天的贮藏实验表明，与黑暗贮藏的样品相比，光贮藏的白菜（ Brassica chinensis L.）叶片中的叶绿素含量更高。对这些样品进行转录组分析以确定光的影响。在 311 个差异表达基因（DEGs）中，早期光诱导蛋白 1（ELIP1）被鉴定为叶绿素合成的主要控制基因。组织和亚细胞定位表明，ELIP1 定位于细胞核中。基序结构分析、染色质免疫沉淀（ChIP）分析、荧光素酶报告基因分析和过表达实验表明，ELIP1 通过结合 G 盒样基序调节基因组非偶联 4（GUN4）、谷氨酰-tRNA 还原酶家族蛋白（HEMA1）和 Mg-原卟啉 IX 甲基转移酶（CHLM）的表达，影响白菜贮藏过程中的叶绿素生物合成。这可能是光合生物中叶绿素、血红素和胆红素色素的共同四吡咯生物合成途径。我们的研究还表明，白光可以作为一种调节因子，提高白菜的贮藏能力和延长货架期。
