Department of Animal Science, University of Nebraska, Lincoln, NE.
J Anim Sci. 2018 Apr 3;96(3):1017-1031. doi: 10.1093/jas/skx034.
Spray-dried porcine plasma (SDPP) has been considered as an alternative for in-feed antibiotics to improve pig growth performance; however, the effect of SDPP on gut microbiota is unknown. The objective of this study was to evaluate effects of feeding SDPP on fecal microbial communities of nursery pigs. Ninety-six weaned pigs were assigned to 16 pens, which were allotted to two dietary treatments, including the control or the control + SDPP (5% and 2.5% SDPP inclusion in phase 1 and 2, respectively) diet. Fecal samples were collected at d 0, 7, 14, 21, and 28. Multiplex sequencing of V3 region of the 16S rRNA gene was used to characterize the bacterial community structure of fecal samples. Pearson's correlation tests were performed in Calypso to identify bacterial taxa that were either positively or negatively associated with overall growth performance. Feeding SDPP altered microbial structure at family, genus, and operational taxonomic unit (OTU) classifications; however, fecal microbes shifted with time. At the family level, Clostridiaceae increased (P < 0.001) on d 14, but decreased (P < 0.05) on d 28 in SDPP-fed pigs compared with control pigs. Decreased Veillonellaceae (P < 0.05; d 14) and Lachnospiraceae (P = 0.001; overall) were observed in SDPP-fed pigs compared with control pigs. Feeding SDPP increased lactic acid-producing bacteria (Lactobacillus delbrueckii, d 7) and cellulolytic bacteria (Ruminococcus albus, d 7; Clostridium thermocellum, d 7 and 14; and Clostridium saccharoperbutylacetonicum/beijerinckii, d 14; and Megasphaera elsdenii, d 21). On d 28, feeding SDPP decreased (P < 0.05) Clostridium difficile compared with control pigs. In conclusion, feeding SDPP altered fecal microbial communities in nursery pigs. The results of this study may provide information to help explain the positive effects associated with feeding SDPP on nutrient digestibility and gut health of nursery pigs.
喷雾干燥猪血浆（SDPP）已被认为是替代饲料中抗生素的一种方法，以提高猪的生长性能；然而，SDPP 对肠道微生物群的影响尚不清楚。本研究旨在评估饲喂 SDPP 对保育猪粪便微生物群落的影响。将 96 头断奶猪分配到 16 个栏中，这些栏被分配到两种日粮处理中，包括对照组或对照组+SDPP（第 1 阶段和第 2 阶段分别添加 5%和 2.5%的 SDPP）日粮。在第 0、7、14、21 和 28 天收集粪便样本。使用 16S rRNA 基因 V3 区的多重测序来描述粪便样本的细菌群落结构。在 Calypso 中进行 Pearson 相关测试，以确定与整体生长性能呈正相关或负相关的细菌分类群。饲喂 SDPP 改变了粪便微生物在科、属和操作分类单元（OTU）分类中的结构；然而，粪便微生物随着时间的推移而变化。在科水平上，与对照组相比，饲喂 SDPP 的猪在第 14 天梭菌科（Clostridiaceae）增加（P < 0.001），但在第 28 天减少（P < 0.05）。与对照组相比，饲喂 SDPP 的猪在第 14 天观察到韦荣氏球菌科（Veillonellaceae）减少（P < 0.05）和lachnospiraceae 减少（P = 0.001；整体）。饲喂 SDPP 增加了产乳酸细菌（乳酸乳球菌，d7）和纤维素分解细菌（毛螺菌科，d7；梭菌 thermocellum，d7 和 14；和梭菌 saccharoperbutylacetonicum/ beijerinckii，d14；和 Megasphaera elsdenii，d21）。在第 28 天，与对照组相比，饲喂 SDPP 减少了艰难梭菌（Clostridium difficile）（P < 0.05）。总之，饲喂 SDPP 改变了保育猪的粪便微生物群落。本研究的结果可能提供信息，有助于解释饲喂 SDPP 对保育猪养分消化率和肠道健康的积极影响。
