Sharifynia Somayeh, Falahati Mehraban, Akhlaghi Lame, Foroumadi Alireza, Fateh Roohollah
Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Mycology and Parasitology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
J Res Med Sci. 2017 Dec 26;22:132. doi: 10.4103/jrms.JRMS_106_17. eCollection 2017.
Rapid and accurate identification and evaluation of antifungal susceptibility pattern of isolates are crucial to determine suitable antifungal drugs for the treatment of patients with vulvovaginitis candidiasis.
Vaginal samples were collected from 150 women with suspicious vaginal candidiasis, and then cultured on Sabouraoud's Dextrose Agar with chloramphenicol to isolate species. After identification of isolates using polymerase chain reaction-restriction fragment length polymorphism technique, antifungal susceptibility testing of four azolic antifungal drugs was carried out using broth microdilution method according to the CLSI M27-A3.
species were isolated from eighty suspected patients (61.79%). The most common pathogen was (63.75%). Resistance to fluconazole and ketoconazole was observed in 27.5% and 23.75% of isolates, respectively, and only 2% of isolates were resistant to miconazole. Interestingly, resistance to fluconazole in was more than other species.
The results indicated that therapy should be selected according to the antifungal susceptibility tests for the prevention of treatment failure and miconazole therapy can be considered as the best therapeutic choice in the management of vulvovaginitis.
快速准确地鉴定和评估分离株的抗真菌药敏模式对于确定治疗外阴阴道念珠菌病患者的合适抗真菌药物至关重要。
从150名疑似阴道念珠菌病的女性中采集阴道样本，然后接种于含氯霉素的沙氏葡萄糖琼脂培养基上进行培养以分离菌种。使用聚合酶链反应-限制性片段长度多态性技术鉴定分离株后，根据CLSI M27-A3采用肉汤微量稀释法对四种唑类抗真菌药物进行抗真菌药敏试验。
从80名疑似患者（61.79%）中分离出菌种。最常见的病原体是（63.75%）。分别有27.5%和23.75%的分离株对氟康唑和酮康唑耐药，仅有2%的分离株对咪康唑耐药。有趣的是，中的氟康唑耐药率高于其他菌种。
结果表明应根据抗真菌药敏试验选择治疗方案以预防治疗失败，咪康唑治疗可被视为外阴阴道炎管理中的最佳治疗选择。
