Gao Fangluan, Shen Jianguo, Liao Furong, Cai Wei, Lin Shuangqing, Yang Hongkai, Chen Shouling
Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Inspection and Quarantine Technology Center, Fujian Exit-Entry Inspection and Quarantine Bureau, Fuzhou, 350001, People's Republic of China.
Arch Virol. 2018 May;163(5):1383-1386. doi: 10.1007/s00705-018-3741-x. Epub 2018 Feb 1.
The complete sequence of a narcissus virus isolated from the Netherlands (Narv-NL) was determined to be 8172 nucleotides in length with an open reading frame encoding for 2624 amino acids. Phylogenetic analysis indicates that Narv-NL is clustered with high confidence among representative members from the genus Macluravirus, including artichoke latent virus (ArLV) and Chinese yam necrotic mosaic virus (CYNMV). Sequence analyses indicated Narv-NL shares 67%-69% nucleotide and 51%-68% amino acid sequence identity with ArLV and CYNMV either in the complete ORF or the coat protein (CP) gene, whereas it had 81%-99 % nucleotide and 80%-99 % amino acid sequence identity with the corresponding CP sequences of narcissus latent virus (NLV) isolates, suggesting that Narv-NL is a member of NLV. To our knowledge, this is the first report of the complete sequence of a NLV isolate.
从荷兰分离出的一种水仙病毒（Narv-NL）的完整序列被确定为8172个核苷酸长，具有一个编码2624个氨基酸的开放阅读框。系统发育分析表明，Narv-NL与马铃薯Y病毒属的代表性成员高度可靠地聚类在一起，包括菊芋潜隐病毒（ArLV）和山药坏死花叶病毒（CYNMV）。序列分析表明，Narv-NL在完整开放阅读框或外壳蛋白（CP）基因中与ArLV和CYNMV的核苷酸序列同一性为67%-69%，氨基酸序列同一性为51%-68%，而它与水仙潜隐病毒（NLV）分离株的相应CP序列的核苷酸序列同一性为81%-99%，氨基酸序列同一性为80%-99%，这表明Narv-NL是NLV的一个成员。据我们所知，这是关于NLV分离株完整序列的首次报道。
