Verdin Anthony, Lounès-Hadj Sahraoui Anissa, Newsam Ray, Robinson Gary, Durand Roger
Laboratoire de Mycologie/Phytopathologie/Environnement, Université du Littoral-Côte d'Opale, 17 avenue Blériot, BP 699, 62228 Calais Cedex, France.
Environ Pollut. 2005 Jan;133(2):283-91. doi: 10.1016/j.envpol.2004.05.040.
Accumulation and elimination of polycyclic aromatic hydrocarbons (PAHs) were studied in the fungus Fusarium solani. When the fungus was grown on a synthetic medium containing benzo[a]pyrene, hyphae of F. solani contained numerous lipid vesicles which could be stained by the lipid-specific dyes: Sudan III and Rhodamine B. The fluorescence produced by Rhodamine B and PAH benzo[a]pyrene were at the same locations in the fungal hyphae, indicating that F. solani stored PAH in pre-existing lipid vesicles. A passive temperature-independent process is involved in the benzo[a]pyrene uptake and storage. Sodium azide, a cytochrome c oxidation inhibitor, and the two cytoskeleton inhibitors colchicine and cytochalasin did not prevent the transport and accumulation of PAH in lipid vesicles of F. solani hyphae. F. solani degraded a large range of PAHs at different rates. PAH intracellular storage in lipid vesicles was not necessarily accompanied by degradation and was common to numerous other fungi.
在茄病镰刀菌中研究了多环芳烃（PAHs）的积累和消除。当该真菌在含有苯并[a]芘的合成培养基上生长时，茄病镰刀菌的菌丝含有大量脂质小泡，这些小泡可以被脂质特异性染料苏丹III和罗丹明B染色。罗丹明B和PAH苯并[a]芘产生的荧光在真菌菌丝中的位置相同，表明茄病镰刀菌将PAH储存在预先存在的脂质小泡中。苯并[a]芘的摄取和储存涉及一个与温度无关的被动过程。叠氮化钠（一种细胞色素c氧化抑制剂）以及两种细胞骨架抑制剂秋水仙碱和细胞松弛素并不能阻止PAH在茄病镰刀菌菌丝脂质小泡中的运输和积累。茄病镰刀菌以不同速率降解多种PAHs。PAH在脂质小泡中的细胞内储存不一定伴随着降解，并且在许多其他真菌中也很常见。
