University of Pittsburgh School of Dental Medicine, Pittsburgh, PA, USA.
Caries Res. 2018;52(4):263-271. doi: 10.1159/000481414. Epub 2018 Jan 25.
Dental caries is a multifactorial infectious disease and a major public health problem estimated to affect 60-90% of school children as well as a vast number of adults. The aim of this work was to define patterns of progression of the disease based on longitudinal data in contrast to using a cross-sectional assessment. dmft/DMFT scores were collected at ages 5, 12, 14, 16, 17, and 18 from 876 individuals. We tested our newly defined phenotypes for association with genetic variants in genes shown to be associated with caries. We generated genotyping data using Taqman chemistry in markers of genes involved in processes such as enamel formation and salivary contributions. Kallikrein 4 (KLK4) was found to show a significant association with the created phenotypes (p = 0.0008 in a recessive model for low caries experience in the primary dentition vs. high caries experience in the primary dentition, and p = 0.0004 in a recessive model for caries free primary dentition vs. high caries experience in the primary dentition).
龋齿是一种多因素的传染病，也是一个主要的公共卫生问题，据估计，它会影响 60-90%的学龄儿童以及大量成年人。这项工作的目的是根据纵向数据来定义疾病的进展模式，而不是使用横断面评估。从 876 个人中收集了 dmft/DMFT 评分，这些人在 5 岁、12 岁、14 岁、16 岁、17 岁和 18 岁时进行了测量。我们测试了我们新定义的表型与已证明与龋齿相关的基因中的遗传变异之间的关联。我们使用 Taqman 化学方法在参与牙釉质形成和唾液贡献等过程的基因的标记物中生成了基因分型数据。Kallikrein 4 (KLK4) 被发现与创建的表型显著相关（在原发性牙齿中低龋齿经历的隐性模型中，与高龋齿经历相比，p = 0.0008，在原发性牙齿中无龋齿经历的隐性模型中，与高龋齿经历相比，p = 0.0004）。
