a Department of Biotechnology , Dayananda Sagar College of Engineering , Bengaluru , India.
b Department of Studies in Microbiology , University of Mysore , Mysore , India.
Artif Cells Nanomed Biotechnol. 2018;46(sup1):676-683. doi: 10.1080/21691401.2018.1434188. Epub 2018 Feb 5.
This research study is to develop an easy and eco-friendly method for the synthesis of AgNPs using aqueous extract of endophytic fungi, Cladosporium species (CsAgNPs) and investigated the effects of antioxidant, anti-diabetic and anti-acetylcholinesterase (AChE), anti-butyrylcholinesterase (BChE) activity. Cladosporium species-mediated silver nanoparticles were characterized by UV-Vis spectrophotometer, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). The aqueous extract of Cladosporium species has shown the presence of carbohydrates, tannin, phenolic glycosides, terpenoids, alkaloids, phenol and anthraquinones. At 438 nm conformed the absorbance of AgNPs. The SEM result confirms that size, morphology and high density of the synthesized nanoparticles with huge disparity in the particle size distribution. The FTIR analysis confirmed the important biological compounds responsible for reduction of silver. Strong absorption property of AgNPs was studied by EDX. In antioxidant activity, CsAgNPs showed the involvement of NADPH-dependent reductase in the formation of AgNPs. The AgNPs has reduced the activity of α-amylase, α-glucosidase and dipeptidyl peptidase IV in vitro antidiabetic activity. The CsAgNPs showed significant glucose uptake in 3T3L1 cell line. The AgNPs have shown excellent inhibitory activity against AChE and BChE. To our best knowledge, this is the first on the synthesis of silver nanoparticles using endophytic fungi, Cladosporium species isolated from healthy leaf of Loranthus micranthus. Hence, to validate our results the in vivo animal studies at molecular level are needed to develop an antioxidant, anti-diabetic and anti-cholinesterase agent.
这项研究旨在开发一种使用内生真菌枝顶孢（Cladosporium species）水提物合成银纳米粒子（CsAgNPs）的简便、环保方法，并研究其抗氧化、抗糖尿病和抗乙酰胆碱酯酶（AChE）、抗丁酰胆碱酯酶（BChE）活性的影响。枝顶孢介导的银纳米粒子通过紫外-可见分光光度计、傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）和能谱（EDX）进行表征。枝顶孢的水提物中含有碳水化合物、单宁、酚糖苷、萜类、生物碱、酚类和蒽醌类物质。在 438nm 处出现了银纳米粒子的吸收峰。SEM 结果证实了合成的纳米粒子具有大小、形态和高密度，且粒径分布差异很大。FTIR 分析证实了参与银还原的重要生物化合物。通过 EDX 研究了 AgNPs 的强吸收特性。在抗氧化活性方面，CsAgNPs 显示 NADPH 依赖性还原酶参与了 AgNPs 的形成。AgNPs 在体外抗糖尿病活性中降低了α-淀粉酶、α-葡萄糖苷酶和二肽基肽酶 IV 的活性。CsAgNPs 在 3T3L1 细胞系中显示出显著的葡萄糖摄取能力。AgNPs 对 AChE 和 BChE 表现出优异的抑制活性。据我们所知，这是首次使用内生真菌枝顶孢从桑寄生健康叶片中合成银纳米粒子。因此，为了验证我们的结果，需要在分子水平上进行体内动物研究，以开发抗氧化、抗糖尿病和抗胆碱酯酶药物。
