Mishra Sushma, Sharma Shilpi
Department of Botany, University of Lucknow, Lucknow, Uttar Pradesh 226007 India.
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi, Delhi 110016 India.
Physiol Mol Biol Plants. 2023 Aug;29(8):1117-1134. doi: 10.1007/s12298-023-01352-2. Epub 2023 Sep 12.
The female spikes (fruits) of are widely used in Ayurvedic, Siddha and Unani medicine systems to treat respiratory and digestive disorders. The spikes are rich in piperine, a pharmacologically active amide alkaloid and a potent bioavailability enhancer, which accumulates to the highest level during the dark-green stage of spike development. Plant-associated microbiota influence the plant's fitness, response, and production of economically important metabolites. Considering the economic importance of piperine and other spike-derived alkaloids, understanding microbial community dynamics during spike development would be key to bioprospecting for economically important metabolites. In the present study, the structural diversity of microbial communities associated with early (SI), mid (SII), and late (SIII) stages of spike development in has been analysed by Illumina-based amplicon sequencing of 16S rRNA gene and ITS region. Results revealed that spike development significantly drives the diversity and abundance of spike-associated microbiota, especially bacterial communities. and constituted the most abundant bacterial and fungal phyla, respectively, across all stages of spike development. Interestingly, and were found to be exclusively associated with SIII (corresponding to economically important) stage of spike development. and constituted the core microbiome of the spike. Besides, PICRUSt analysis revealed that amino acid metabolism was the most dominant metabolic function attributed to spike-associated bacterial communities. To the best of our knowledge, this is the first study to investigate the endomicrobiome dynamics during spike development in a medicinal plant species.
The online version contains supplementary material available at 10.1007/s12298-023-01352-2.
[植物名称]的雌花序（果实）在阿育吠陀、悉达和尤那尼医学体系中被广泛用于治疗呼吸和消化系统疾病。雌花序富含胡椒碱，一种具有药理活性的酰胺生物碱和强效生物利用度增强剂，其在雌花序发育的深绿色阶段积累至最高水平。与植物相关的微生物群会影响植物的健康、反应以及经济上重要的代谢产物的产生。考虑到胡椒碱和其他来自雌花序的生物碱的经济重要性，了解雌花序发育过程中的微生物群落动态对于经济上重要的代谢产物的生物勘探至关重要。在本研究中，通过基于Illumina的16S rRNA基因和ITS区域扩增子测序，分析了[植物名称]雌花序发育早期（SI）、中期（SII）和晚期（SIII）阶段相关微生物群落的结构多样性。结果表明，雌花序发育显著驱动了与雌花序相关的微生物群的多样性和丰度，尤其是细菌群落。[细菌门名称]和[真菌门名称]分别是雌花序发育所有阶段中最丰富的细菌和真菌门。有趣的是，[细菌属名称1]和[细菌属名称2]被发现仅与雌花序发育的SIII阶段（对应于经济上重要的阶段）相关。[细菌属名称3]和[细菌属名称4]构成了雌花序的核心微生物群。此外，PICRUSt分析表明，氨基酸代谢是与雌花序相关细菌群落最主要的代谢功能。据我们所知，这是第一项研究药用植物物种雌花序发育过程中内生微生物群动态的研究。
在线版本包含可在10.1007/s12298-023-01352-2获取的补充材料。
