Asai Yasutsugu, Itoi Takao, Sugimoto Masahiro, Sofuni Atsushi, Tsuchiya Takayoshi, Tanaka Reina, Tonozuka Ryosuke, Honjo Mitsuyoshi, Mukai Shuntaro, Fujita Mitsuru, Yamamoto Kenjiro, Matsunami Yukitoshi, Kurosawa Takashi, Nagakawa Yuichi, Kaneko Miku, Ota Sana, Kawachi Shigeyuki, Shimazu Motohide, Soga Tomoyoshi, Tomita Masaru, Sunamura Makoto
Division of Gastroenterology and Hepatology, Tokyo Medical University, Shinjuku, Tokyo 160-0023, Japan.
Research and Development Center for Minimally Invasive Therapies Health Promotion and Preemptive Medicine, Tokyo Medical University, Shinjuku, Tokyo 160-8402, Japan.
Cancers (Basel). 2018 Feb 5;10(2):43. doi: 10.3390/cancers10020043.
Detection of pancreatic cancer (PC) at a resectable stage is still difficult because of the lack of accurate detection tests. The development of accurate biomarkers in low or non-invasive biofluids is essential to enable frequent tests, which would help increase the opportunity of PC detection in early stages. Polyamines have been reported as possible biomarkers in urine and saliva samples in various cancers. Here, we analyzed salivary metabolites, including polyamines, using capillary electrophoresis-mass spectrometry. Salivary samples were collected from patients with PC ( = 39), those with chronic pancreatitis (CP, = 14), and controls (C, = 26). Polyamines, such as spermine, ₁-acetylspermidine, and ₁-acetylspermine, showed a significant difference between patients with PC and those with C, and the combination of four metabolites including ₁-acetylspermidine showed high accuracy in discriminating PC from the other two groups. These data show the potential of saliva as a source for tests screening for PC.
由于缺乏准确的检测方法，在可切除阶段检测胰腺癌（PC）仍然很困难。在低侵入性或非侵入性生物流体中开发准确的生物标志物对于实现频繁检测至关重要，这将有助于增加早期检测PC的机会。多胺已被报道为各种癌症尿液和唾液样本中可能的生物标志物。在这里，我们使用毛细管电泳-质谱分析了包括多胺在内的唾液代谢物。从胰腺癌患者（n = 39）、慢性胰腺炎患者（CP，n = 14）和对照组（C，n = 26）中收集唾液样本。精胺、N1-乙酰亚精胺和N1-乙酰精胺等多胺在胰腺癌患者和对照组之间存在显著差异，包括N1-乙酰亚精胺在内的四种代谢物组合在区分胰腺癌与其他两组方面具有很高的准确性。这些数据表明唾液作为PC检测筛查来源的潜力。
