龙血树树脂甲醇提取物对大鼠的急性和亚急性经口毒性
Acute and sub-acute oral toxicity of Dracaena cinnabari resin methanol extract in rats.
作者信息
Al-Afifi Nashwan Abdullah, Alabsi Aied Mohammed, Bakri Marina Mohd, Ramanathan Anand
机构信息
Department of Oral and Craniofacial Sciences, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, MAHSA University, 42610, Jenjarom, Selangor, Malaysia.
出版信息
BMC Complement Altern Med. 2018 Feb 5;18(1):50. doi: 10.1186/s12906-018-2110-3.
BACKGROUND
Dracaena cinnabari (DC) is a perennial tree that located on the Southern coast of Yemen native to the Socotra Island. This tree produces a deep red resin known as the Dragon's blood, the Twobrother's Blood or Damm Alakhwain. The current study performed to evaluate the safety of the DC resin methanol extract after a single or 28 consecutive daily oral administrations.
METHODS
In assessing the safety of DC resin methanol extract, acute and sub-acute oral toxicity tests performed following OECD guidelines 423 and 407, respectively, with slight modifications. In acute oral toxicity test, DC resin methanol extract administered to female Sprague Dawley rats by oral gavage at a single dose of 300 and 2000 mg/kg body weight. Rats observed for toxic signs for 14 days. In sub-acute oral toxicity test, DC resin methanol extract administered to the rats by oral gavage at 500, 1000, and 1500 mg/kg body weight daily up to 28 days to male and female Spradgue Dawley rats. The control and high dose in satellite groups were also maintained and handled as the previous groups to determine the late onset toxicity of DC resin methanol extract. At the end of each test, hematological and biochemical analysis of the collected blood were performed as well as gross and microscopic pathology.
RESULTS
In acute oral toxicity, no treatment-related death or toxic signs were observed. It revealed that the DC resin methanol extract could be well tolerated up to the dose 2000 mg/kg body weight and could be classified as Category 5. The sub-acute test observations indicated that there are no treatment-related changes up to the high dose level compared to the control. Food consumption, body weight, organ weight, hematological parameters, biochemical parameters and histopathological examination (liver, kidney, heart, spleen and lung) revealed no abnormalities. Water intake was significantly higher in the DC resin methanol extract treated groups compared to the control.
CONCLUSION
This study demonstrates tolerability of DC resin methanol extract administered daily for 28 days up to 1500 mg/kg dose.
背景
龙血树是一种多年生树木，生长在也门南部海岸的索科特拉岛。这种树会分泌一种深红色树脂，被称为龙血、双兄弟之血或达玛阿拉赫万。本研究旨在评估龙血树树脂甲醇提取物单次或连续28天每日口服给药后的安全性。
方法
在评估龙血树树脂甲醇提取物的安全性时，分别按照经合组织准则423和407进行急性和亚急性口服毒性试验，并做了轻微修改。在急性口服毒性试验中，通过灌胃法给雌性斯普拉格-道利大鼠单次口服300和2000毫克/千克体重的龙血树树脂甲醇提取物。观察大鼠14天的中毒症状。在亚急性口服毒性试验中，给雄性和雌性斯普拉格-道利大鼠每日经口灌胃500、1000和1500毫克/千克体重的龙血树树脂甲醇提取物，持续28天。卫星组的对照组和高剂量组也按照之前的组进行维持和处理，以确定龙血树树脂甲醇提取物的迟发性毒性。每次试验结束时，对采集的血液进行血液学和生化分析以及大体和显微镜病理学检查。
结果
在急性口服毒性试验中，未观察到与治疗相关的死亡或中毒症状。结果表明，龙血树树脂甲醇提取物在高达2000毫克/千克体重的剂量下耐受性良好，可归类为第5类。亚急性试验观察表明，与对照组相比，在高剂量水平以下没有与治疗相关的变化。食物摄入量、体重、器官重量、血液学参数、生化参数和组织病理学检查（肝脏、肾脏、心脏、脾脏和肺）均未发现异常。与对照组相比，龙血树树脂甲醇提取物处理组的水摄入量显著更高。
结论
本研究表明，龙血树树脂甲醇提取物在高达1500毫克/千克剂量下每日给药28天具有耐受性。
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