Qi Xinpeng, Li Man-Wah, Xie Min, Liu Xin, Ni Meng, Shao Guihua, Song Chi, Kay-Yuen Yim Aldrin, Tao Ye, Wong Fuk-Ling, Isobe Sachiko, Wong Chi-Fai, Wong Kwong-Sen, Xu Chunyan, Li Chunqing, Wang Ying, Guan Rui, Sun Fengming, Fan Guangyi, Xiao Zhixia, Zhou Feng, Phang Tsui-Hung, Liu Xuan, Tong Suk-Wah, Chan Ting-Fung, Yiu Siu-Ming, Tabata Satoshi, Wang Jian, Xu Xun, Lam Hon-Ming
1] School of Life Sciences and Center for Soybean Research of the State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin HKSAR, Hong Kong [2].
1] BGI-Shenzhen, Shenzhen 518083, PR China [2].
Nat Commun. 2014 Jul 9;5:4340. doi: 10.1038/ncomms5340.
Using a whole-genome-sequencing approach to explore germplasm resources can serve as an important strategy for crop improvement, especially in investigating wild accessions that may contain useful genetic resources that have been lost during the domestication process. Here we sequence and assemble a draft genome of wild soybean and construct a recombinant inbred population for genotyping-by-sequencing and phenotypic analyses to identify multiple QTLs relevant to traits of interest in agriculture. We use a combination of de novo sequencing data from this work and our previous germplasm re-sequencing data to identify a novel ion transporter gene, GmCHX1, and relate its sequence alterations to salt tolerance. Rapid gain-of-function tests show the protective effects of GmCHX1 towards salt stress. This combination of whole-genome de novo sequencing, high-density-marker QTL mapping by re-sequencing and functional analyses can serve as an effective strategy to unveil novel genomic information in wild soybean to facilitate crop improvement.
采用全基因组测序方法探索种质资源可作为作物改良的一项重要策略，特别是在研究野生种质时，这些野生种质可能含有在驯化过程中丢失的有用遗传资源。在此，我们对野生大豆基因组进行测序和组装，构建一个重组自交群体用于简化基因组测序和表型分析，以鉴定多个与农业相关性状的数量性状基因座（QTL）。我们结合这项工作中的从头测序数据和之前种质重测序数据，鉴定出一个新的离子转运基因GmCHX1，并将其序列改变与耐盐性相关联。快速功能获得性试验表明GmCHX1对盐胁迫具有保护作用。这种全基因组从头测序、基于重测序的高密度标记QTL定位和功能分析相结合的方法，可作为揭示野生大豆新基因组信息以促进作物改良的有效策略。
