In vitro biofilm formation by isolated from wounds of hospital-admitted patients and their association with antimicrobial resistance.

INTRODUCTION

including methicillin-resistant (MRSA) has the propensity to form biofilms, and causes significant mortality and morbidity in the patients with wounds. Our aim was to study the in vitro biofilm-forming ability of isolated from wounds of hospitalized patients and their association with antimicrobial resistance.

MATERIALS AND METHODS

Forty-three clinical isolates of were obtained from 150 pus samples using standard microbiological techniques. Biofilm formation in these isolates was detected by tissue culture plate (TCP) method and tube adherence method (TM). Antimicrobial susceptibility test was performed using the modified Kirby-Bauer disk diffusion method as per Clinical and Laboratory Standards Institute guidelines. MRSA was detected using the cefoxitin disk test.

RESULTS

Biofilm formation was observed in 30 (69.8%) and 28 (65.1%) isolates of via TCP method and TM, respectively. Biofilm-producing exhibited a higher incidence of antimicrobial resistance when compared with the biofilm nonproducers (<0.05). Importantly, 86.7% of biofilm-producing were multidrug resistant (MDR), whereas all the biofilm nonproducers were non-MDR (<0.05). Large proportions (43.3%) of biofilm producers were identified as MRSA; however, none of the biofilm nonproducers were found to be MRSA (<0.05).

CONCLUSION

Both the in vitro methods showed that isolated from wound infection of hospitalized patients have high degree of biofilm-forming ability. Biofilm-producing strains have very high tendency to exhibit antimicrobial resistance, multidrug resistance and methicillin resistance. Regular surveillance of biofilm formation by and their antimicrobial resistance profile may lead to the early treatment of the wound infection.