Neopane Puja, Nepal Hari Prasad, Shrestha Rojeet, Uehara Osamu, Abiko Yoshihiro
Department of Microbiology, Chitwan Medical College and Teaching Hospital, Bharatpur, Nepal.
Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Japan.
Int J Gen Med. 2018 Jan 18;11:25-32. doi: 10.2147/IJGM.S153268. eCollection 2018.
including methicillin-resistant (MRSA) has the propensity to form biofilms, and causes significant mortality and morbidity in the patients with wounds. Our aim was to study the in vitro biofilm-forming ability of isolated from wounds of hospitalized patients and their association with antimicrobial resistance.
Forty-three clinical isolates of were obtained from 150 pus samples using standard microbiological techniques. Biofilm formation in these isolates was detected by tissue culture plate (TCP) method and tube adherence method (TM). Antimicrobial susceptibility test was performed using the modified Kirby-Bauer disk diffusion method as per Clinical and Laboratory Standards Institute guidelines. MRSA was detected using the cefoxitin disk test.
Biofilm formation was observed in 30 (69.8%) and 28 (65.1%) isolates of via TCP method and TM, respectively. Biofilm-producing exhibited a higher incidence of antimicrobial resistance when compared with the biofilm nonproducers (<0.05). Importantly, 86.7% of biofilm-producing were multidrug resistant (MDR), whereas all the biofilm nonproducers were non-MDR (<0.05). Large proportions (43.3%) of biofilm producers were identified as MRSA; however, none of the biofilm nonproducers were found to be MRSA (<0.05).
Both the in vitro methods showed that isolated from wound infection of hospitalized patients have high degree of biofilm-forming ability. Biofilm-producing strains have very high tendency to exhibit antimicrobial resistance, multidrug resistance and methicillin resistance. Regular surveillance of biofilm formation by and their antimicrobial resistance profile may lead to the early treatment of the wound infection.
包括耐甲氧西林金黄色葡萄球菌（MRSA）在内的金黄色葡萄球菌易于形成生物膜，并导致伤口患者出现显著的死亡率和发病率。我们的目的是研究从住院患者伤口分离出的金黄色葡萄球菌的体外生物膜形成能力及其与抗菌药物耐药性的关系。
使用标准微生物学技术从150份脓液样本中获得43株临床分离的金黄色葡萄球菌。通过组织培养板（TCP）法和试管黏附法（TM）检测这些分离株中的生物膜形成情况。按照临床和实验室标准协会指南，使用改良的 Kirby-Bauer 纸片扩散法进行抗菌药物敏感性试验。使用头孢西丁纸片试验检测 MRSA。
通过TCP法和TM法分别在30株（69.8%）和28株（65.1%）金黄色葡萄球菌分离株中观察到生物膜形成。与不产生生物膜的菌株相比，产生生物膜的金黄色葡萄球菌表现出更高的抗菌药物耐药发生率（P<0.05）。重要的是，86.7%产生生物膜的金黄色葡萄球菌为多重耐药（MDR），而所有不产生生物膜的菌株均非MDR（P<0.05）。很大比例（43.3%）的生物膜产生菌被鉴定为MRSA；然而，未发现任何不产生生物膜的菌株为MRSA（P<0.05）。
两种体外方法均显示，从住院患者伤口感染中分离出的金黄色葡萄球菌具有高度的生物膜形成能力。产生生物膜的菌株表现出抗菌药物耐药、多重耐药和耐甲氧西林的倾向非常高。定期监测金黄色葡萄球菌的生物膜形成及其抗菌药物耐药谱可能有助于伤口感染的早期治疗。
