Xie Hao-Yue, Wang Zuo-Rong, Fu Zhi-Feng
Key Laboratory of Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Zigong Institute for Food and Drug Control, Zigong 643010, China.
J Pharm Anal. 2014 Dec;4(6):412-416. doi: 10.1016/j.jpha.2014.05.004. Epub 2014 May 27.
A simple, fast and sensitive capillary electrophoresis (CE) strategy combined with chemiluminescence (CL) detection for analysis of ofloxacin (OF) enantiomers was established in the present work. Sulfonated β-cyclodextrin (β-CD) was used as the chiral additive being added into the running buffer of luminol-diperiodatocuprate (III) (K[Cu(HIO)], DPC) chemiluminescence system. Under the optimum conditions, the proposed method was successfully applied to separation and analysis of OF enantiomers with the detection limits (/=3) of 8.0 nM and 7.0 nM for levofloxacin and dextrofloxacin, respectively. The linear ranges were both 0.010-100 μM. The method was utilized for analyzing OF in urine; the results obtained were satisfactory and recoveries were 89.5-110.8%, which demonstrated the reliability of this method. This approach can also be further extended to analyze different commercial OF medicines.
本研究建立了一种简单、快速且灵敏的毛细管电泳（CE）结合化学发光（CL）检测法用于分析氧氟沙星（OF）对映体。磺化β-环糊精（β-CD）用作手性添加剂，添加到鲁米诺-二高碘酸铜（III）（K[Cu(HIO)]，DPC）化学发光体系的运行缓冲液中。在最佳条件下，该方法成功应用于OF对映体的分离分析，左氧氟沙星和右氧氟沙星的检测限（/=3）分别为8.0 nM和7.0 nM。线性范围均为0.010 - 100 μM。该方法用于尿液中OF的分析；所得结果令人满意，回收率为89.5 - 110.8%，证明了该方法的可靠性。该方法还可进一步扩展用于分析不同的市售OF药物。
