Marashi Seyed Mahmoud Amin, Moulana Zahra, Imani Fooladi Abbas Ali, Mashhadi Karim Mohammad
Department of Microbiology and Immunology, Alborz University of Medical Sciences, Karaj, IR Iran.
Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, IR Iran.
Jundishapur J Microbiol. 2014 Apr;7(4):e9450. doi: 10.5812/jjm.9450. Epub 2014 Apr 1.
For a long time, infertility has been one of the most sequels in medical sciences with microbial agents as one group of its causes. The possible etiological role of Chlamydia trachomatis in infertility was suggested years ago, but it has not yet been proved completely. To decrease the severe involvements of C. trachomatis infections, screening by efficient diagnostic methods are necessary.
In this study we attempted to determine the incidence of C. trachomatis in infertile women and compared this with healthy women.
This case-control study was performed on 150 infertile women with unknown causes and without physiological deficiency for infertility. The control group consisted of 200 fertile safe and impregnated women. Presence of C. trachomatis in the two groups was examined by direct and indirect immunofluorescence tests and PCR.
C. trachomatis was detected by direct immunofluorescence method in 23 (15.3%) infertile women compared and 7 (3.5%) healthy controls. Using indirect immunofluorescence tests, a positive test titer of 1:16 as well as the above results were detected in 34 (22.6%) of the infertile cases and 9 (4.5%) of the controls. C. trachomatis was detected by PCR method in 48 (32%) infertile women and 13 (8.7%) among the controls.
The results of our study suggest that there is a significant association between C. trachomatis infection and female infertility.
长期以来，不孕症一直是医学领域最常见的后遗症之一，微生物因素是其病因之一。数年前就有人提出沙眼衣原体在不孕症中可能的病因作用，但尚未得到充分证实。为减少沙眼衣原体感染的严重影响，采用有效的诊断方法进行筛查很有必要。
在本研究中，我们试图确定不孕女性中沙眼衣原体的发病率，并与健康女性进行比较。
本病例对照研究对150名原因不明且无生理性不孕缺陷的不孕女性进行。对照组由200名可育、健康且已受孕的女性组成。通过直接和间接免疫荧光试验及聚合酶链反应检测两组中沙眼衣原体的存在情况。
通过直接免疫荧光法，在23名（15.3%）不孕女性中检测到沙眼衣原体，而健康对照组中有7名（3.5%）检测到。使用间接免疫荧光试验，在34名（22.6%）不孕病例中检测到阳性滴度为1:16的结果，对照组中有9名（4.5%）。通过聚合酶链反应法，在48名（32%）不孕女性中检测到沙眼衣原体，对照组中有13名（8.7%）。
我们的研究结果表明，沙眼衣原体感染与女性不孕之间存在显著关联。
