Rivadeneira Josefina, Audisio M C, Gorustovich Alejandro
1 Interdisciplinary Materials Group-IESIING-UCASAL, INTECIN UBA-CONICET, Salta, Argentina.
2 Instituto de Investigaciones para la Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Salta, Argentina.
J Biomater Appl. 2018 Apr;32(9):1231-1238. doi: 10.1177/0885328218756653. Epub 2018 Feb 6.
No single material can provide all requirements for wound dressings. Here, we evaluated the influence of different soy protein isolate and agar proportions (3:1, 1:1, and 1:3) in blend films on some of their physical-chemical and antibacterial properties to elucidate their potential as wound dressings. The films were synthesized by the gel casting method and ciprofloxacin hydrochloride was incorporated into the films. Films were characterized based on their surface morphology, water uptake ability, and weight loss profile. Also, the ciprofloxacin hydrochloride release kinetics was quantified spectrophotometrically. The antibacterial effect was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa strains. The soy protein isolate-agar ratio affected the water uptake of the films and the release profile of ciprofloxacin hydrochloride but not the weight loss profile. The amount of drug released decreased near 80% because of the decrease in agar content in the films. The release kinetics of ciprofloxacin hydrochloride data best fitted to the Korsmeyer-Peppas model, suggesting that the mechanism of drug release was mainly of the diffusion type. All ciprofloxacin hydrochloride-releasing soy protein isolate-agar films strongly inhibited the cell viability of the bacterial strains studied. We concluded that water uptake and ciprofloxacin hydrochloride release can be controlled by changing the soy protein isolate-agar proportion. The proportions did not lead to changes in the antibacterial strength of the films.
没有单一材料能够满足伤口敷料的所有要求。在此，我们评估了共混膜中不同大豆分离蛋白与琼脂比例（3:1、1:1和1:3）对其一些物理化学和抗菌性能的影响，以阐明它们作为伤口敷料的潜力。通过凝胶浇铸法合成薄膜，并将盐酸环丙沙星掺入薄膜中。基于薄膜的表面形态、吸水能力和失重曲线对薄膜进行表征。此外，用分光光度法定量盐酸环丙沙星的释放动力学。评估了对金黄色葡萄球菌和铜绿假单胞菌菌株的抗菌效果。大豆分离蛋白与琼脂的比例影响薄膜的吸水能力和盐酸环丙沙星的释放曲线，但不影响失重曲线。由于薄膜中琼脂含量的降低，药物释放量减少了近80%。盐酸环丙沙星的数据释放动力学最符合Korsmeyer-Peppas模型，表明药物释放机制主要为扩散型。所有释放盐酸环丙沙星的大豆分离蛋白-琼脂薄膜都强烈抑制了所研究细菌菌株的细胞活力。我们得出结论，通过改变大豆分离蛋白-琼脂的比例可以控制吸水和盐酸环丙沙星的释放。这些比例并未导致薄膜抗菌强度的变化。
