Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, PR China; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, PR China.
Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Int J Biol Macromol. 2018 Sep;116:831-839. doi: 10.1016/j.ijbiomac.2018.01.165. Epub 2018 Feb 1.
A purified polysaccharide, designated CALB-4, was acquired from Fructus aurantii that is the traditional edible/medicina plant in China. The present study was performed to characterize the CALB-4 and to evaluate its immunomodulatory activities on human peripheral blood mononuclear cells (PBMCs). The structure of CALB-4 was characterized by partial acid hydrolysis, periodate oxidation, Smith degradation, and methylation analysis combined with gas chromatography-mass spectrometry (GC-MS), Infrared Spectroscopy (IR) and scanning electron microscopy (SEM). The results indicated that CALB-4 was elucidated as a pectic polysaccharide and its main chain is composed of Man, Gal UA and Gal, interspersed with Ara, Rha, Man and Gal. Furthermore, immunological tests showed that CALB-4 exhibits the immunoenhancement effects. The mechanism for this action might be attributed to the increase of the cytoplasmic concentration of pro-IL-1 via the up-regulation of several mitogen-activated protein kinases (MAPKs) and the nuclear translocation of p65. This study clarified that CALB-4 could be as an efficacious biological response modifier in immunotherapy.
从中国传统食用/药用植物枳实中提取出一种纯化多糖，命名为 CALB-4。本研究旨在对 CALB-4 进行表征，并评估其对人外周血单个核细胞 (PBMCs) 的免疫调节活性。通过部分酸水解、高碘酸氧化、Smith 降解和甲基化分析结合气相色谱-质谱联用 (GC-MS)、红外光谱 (IR) 和扫描电子显微镜 (SEM) 对 CALB-4 的结构进行了表征。结果表明，CALB-4 被阐明为一种果胶多糖，其主链由 Man、GalUA 和 Gal 组成，间插有 Ara、Rha、Man 和 Gal。此外，免疫试验表明 CALB-4 具有免疫增强作用。这种作用的机制可能归因于通过上调几种丝裂原活化蛋白激酶 (MAPKs) 和核转位 p65，增加前白细胞介素-1 (pro-IL-1) 的细胞质浓度。本研究阐明了 CALB-4 可以作为免疫治疗中的一种有效的生物反应调节剂。
