甜樱桃（Prunus avium）果胶多糖的特性及其免疫增强活性。
Characteristics and immune-enhancing activity of pectic polysaccharides from sweet cherry (Prunus avium).
机构信息
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, PR China; Taizhou Academy of Agricultural Sciences, Linhai 317000, PR China.
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, PR China.
出版信息
Food Chem. 2018 Jul 15;254:47-54. doi: 10.1016/j.foodchem.2018.01.145. Epub 2018 Jan 31.
Two water soluble polysaccharides components PAPS-1 and PAPS-2 with homogeneously distributed molecular weight were obtained from Prunus avium. PAPS-1 and PAPS-2 contained GalA: Ara: Gal: Rha: GluA: Glu in 49.38: 32.39: 10.68: 4.66: 1.94: 0.48 and 77.18: 14.91: 3.39: 3.46: 0.93: 0.19 M ratios respectively, as well as trace amount of mannose and fucose. Infrared spectroscopy (IR), nuclear magnetic resonance (NMR) and methylation analysis indicated that both fractions were type I rhamnogalacturonan (RG-I) pectic polysaccharides with glycan side chains constituted mainly of arabinose with minor amount of galactose. Galacturonic acid methylation and sugar acetylation was found in both PAPS-1 and PAPS-2. Both PAPS-1 and PAPS-2 significantly induced the NO release from RAW264.7 cells and the expression of several immune-related molecular (TNFα, IL6, IL10, GCSF, iNOS, COX-2) was induced in RAW264.7 cells.
从樱桃李中得到了两种水溶性多糖成分 PAPS-1 和 PAPS-2，它们的分子量分布均匀。PAPS-1 和 PAPS-2 中分别含有 49.38:32.39:10.68:4.66:1.94:0.48 和 77.18:14.91:3.39:3.46:0.93:0.19 的 GalA:Ara:Gal:Rha:GluA:Glu 摩尔比，以及少量的甘露糖和岩藻糖。红外光谱（IR）、核磁共振（NMR）和甲基化分析表明，这两种多糖均为 I 型鼠李半乳糖醛酸聚糖（RG-I）果胶多糖，糖侧链主要由阿拉伯糖组成，少量由半乳糖组成。在 PAPS-1 和 PAPS-2 中均发现了半乳糖醛酸甲酯化和糖乙酰化。PAPS-1 和 PAPS-2 均能显著诱导 RAW264.7 细胞释放 NO，并诱导 RAW264.7 细胞中几种免疫相关分子（TNFα、IL6、IL10、GCSF、iNOS、COX-2）的表达。
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