Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangaluru, India.
Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangaluru, India.
Int J Infect Dis. 2018 Mar;68:74-76. doi: 10.1016/j.ijid.2018.01.018. Epub 2018 Feb 1.
Vibrio vulnificus is a Gram-negative, opportunistic human pathogen capable of causing life-threatening septicaemia, wound infections, and gastroenteritis, especially in immunocompromised individuals. Two cases of V. vulnificus-associated wound infection occurring in diabetic patients are reported here. The pathogen was detected by PCR targeting species-specific marker gyrB and virulence markers, including repeats in toxin (rtxA) and hemolysin (vvhA), but the causative agent could not be cultured. Genotyping based on the virulence-correlated gene revealed that the V. vulnificus detected in this study belonged to the vcg-C type, which is commonly associated with clinical cases. This report highlights the clinical applicability of PCR-based methods in the detection of V. vulnificus in culture-negative cases. Such methods may add a very useful clinical dimension to currently used diagnostic practices.
创伤弧菌是一种革兰氏阴性、机会性人类病原体，能够导致危及生命的败血症、伤口感染和肠胃炎，特别是在免疫功能低下的个体中。本文报告了两例发生在糖尿病患者中的创伤弧菌相关伤口感染病例。通过针对种特异性标记基因 gyB 和毒力标记基因，包括重复毒素 (rtxA) 和溶血素 (vvhA) 的 PCR 检测到了病原体，但无法培养出致病因子。基于与毒力相关的基因进行基因分型表明，本研究中检测到的创伤弧菌属于 vcg-C 型，该型通常与临床病例相关。本报告强调了基于 PCR 的方法在培养阴性病例中检测创伤弧菌的临床适用性。这些方法可能为目前使用的诊断实践增加非常有用的临床维度。
