Department of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.
Pharm Res. 2018 Feb 6;35(3):48. doi: 10.1007/s11095-017-2288-y.
To determine whether formulations containing ceramides (including a ceramide with a long hydroxyl acyl chain linked to a linoleate, CER EOS) and fatty acids are able to repair the skin barrier by normalizing the lipid organization in stratum corneum (SC).
The formulations were applied on a skin barrier repair model consisting of ex vivo human skin from which SC was removed by stripping. The effect of formulations on the lipid organization and conformational ordering in the regenerated SC were analyzed using Fourier transform infrared spectroscopy and small angle X-ray diffraction.
Application of the formulation containing only one ceramide on regenerating SC resulted in a higher fraction of lipids adopting an orthorhombic organization. A similar fraction of lipids forming an orthorhombic organization was observed after application of a formulation containing two ceramides and a fatty acid on regenerating SC. No effects on the lamellar lipid organization were observed.
Application of a formulation containing either a single ceramide or two ceramides and a fatty acid on regenerating SC, resulted in a denser lateral lipid packing of the SC lipids in compromised skin. The strongest effect was observed after application of a formulation containing a single ceramide.
确定是否含有神经酰胺的配方（包括与亚油酸相连的长羟基酰基链的神经酰胺，CER EOS）和脂肪酸能够通过使角质层（SC）中的脂质组织正常化来修复皮肤屏障。
将配方应用于由去除 SC 的体外人皮肤组成的皮肤屏障修复模型。使用傅立叶变换红外光谱和小角 X 射线衍射分析配方对再生 SC 中脂质组织和构象有序性的影响。
仅含有一种神经酰胺的配方应用于再生 SC 导致更高比例的脂质采用正交组织。在再生 SC 上应用含有两种神经酰胺和一种脂肪酸的配方后，观察到形成正交组织的脂质比例相似。未观察到对层状脂质组织的影响。
在再生 SC 上应用含有单一神经酰胺或两种神经酰胺和一种脂肪酸的配方，导致受损皮肤 SC 脂质的侧向脂质堆积更加致密。应用含有单一神经酰胺的配方观察到最强的效果。
