L-乳酸和丙酮酸对细胞毒性T淋巴细胞活化的调节

Regulation of cytotoxic T-lymphocyte activation by L-lactate and pyruvate.

作者信息

Mihm S, Dröge W

出版信息

Cell Immunol. 1985 Nov;96(1):235-40. doi: 10.1016/0008-8749(85)90355-7.

Abstract

The activation of cytotoxic T lymphocytes (CTL) in vivo was found to be strongly augmented by two injections of 0.2 ml 1 X 10(-1) M pyruvate in spite of the relatively high concentration of glucose (approximately 10(-2) M) in the blood. The repeated injection of 1 X 10(-1) M L-lactate, in contrast, was found to suppress cytotoxic responses in vivo. The activation of CTL and DNA synthesis in mixed lymphocyte cultures, on the other hand, was found to be suppressed by pyruvate (1 X 10(-2) M), and was substantially augmented by 1 X 10(-2) M L-lactate. The glucose concentration in the culture medium was also approximately 10(-2) M. Taken together, these results suggest that the utilization of glucose is relatively ineffective and that the respiratory metabolism is a more effective source of energy during the early T cell activation. The results suggest also that the aerobic glycolysis of macrophages and their release of L-lactate may contribute to their function as accessory cells in immune responses. The differences between the in vivo and in vitro results are discussed.

摘要

尽管血液中葡萄糖浓度相对较高（约10⁻² M），但经两次注射0.2 ml 1×10⁻¹ M丙酮酸后，体内细胞毒性T淋巴细胞（CTL）的激活被发现显著增强。相反，重复注射1×10⁻¹ M L-乳酸被发现会抑制体内的细胞毒性反应。另一方面，在混合淋巴细胞培养中，CTL的激活和DNA合成被发现会受到丙酮酸（1×10⁻² M）的抑制，并被1×10⁻² M L-乳酸显著增强。培养基中的葡萄糖浓度也约为10⁻² M。综合来看，这些结果表明葡萄糖的利用相对低效，并且在早期T细胞激活过程中呼吸代谢是更有效的能量来源。结果还表明巨噬细胞的有氧糖酵解及其L-乳酸的释放可能有助于其作为免疫反应中辅助细胞的功能。文中讨论了体内和体外结果之间的差异。