猫孢子丝菌病：里约热内卢动物疫区临床流行病学特征与病原体表型-基因型特征之间的关联
Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area.
作者信息
Boechat Jéssica Sepulveda, Oliveira Manoel Marques Evangelista, Almeida-Paes Rodrigo, Gremião Isabella Dib Ferreira, Machado Ana Caroline de Sá, Oliveira Raquel de Vasconcelos Carvalhaes, Figueiredo Anna Barreto Fernandes, Rabello Vanessa Brito de Souza, Silva Karoline Benevides de Lima, Zancopé-Oliveira Rosely Maria, Schubach Tânia Maria Pacheco, Pereira Sandro Antonio
机构信息
Fundação Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Micologia, Rio de Janeiro, RJ, Brasil.
出版信息
Mem Inst Oswaldo Cruz. 2018 Mar;113(3):185-196. doi: 10.1590/0074-02760170407.
BACKGROUND
Sporotrichosis is caused by species of the genus Sporothrix. From 1998 to 2015, 4,703 cats were diagnosed at the Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil. Even after the description of the Sporothrix species, the characterisation of feline isolates is not performed routinely.
OBJECTIVES
To characterise the clinical isolates from cats at the species level and correlate them with the clinical and epidemiological characteristics of the cats.
METHODS
Forty seven Sporothrix spp. isolates from cats assisted at Fiocruz from 2010 to 2011 were included. Medical records were consulted to obtain the clinical and epidemiological data. The isolates were identified through their morphological and physiological characteristics. T3B polymerase chain reaction (PCR) fingerprinting was used for molecular identification of the species.
FINDINGS
In phenotypic tests, 34 isolates were characterised as S. brasiliensis, one as S. schenckii and 12 as Sporothrix spp. PCR identified all isolates as S. brasiliensis.
MAIN CONCLUSIONS
S. brasiliensis is the only etiological agent of feline sporotrichosis in Rio de Janeiro to date. None association was found between the isolates and the clinical and epidemiological data. In addition, we strongly recommend the use of molecular techniques for the identification of isolates of Sporothrix spp.
背景
孢子丝菌病由孢子丝菌属的物种引起。1998年至2015年期间，巴西里约热内卢的奥斯瓦尔多·克鲁兹基金会（Fiocruz）诊断出4703只猫感染此病。即便在孢子丝菌物种被描述之后，猫分离株的特征鉴定也并非常规操作。
目的
在物种水平上对猫的临床分离株进行特征鉴定，并将其与猫的临床和流行病学特征相关联。
方法
纳入了2010年至2011年在Fiocruz接受治疗的47株来自猫的孢子丝菌属分离株。查阅病历以获取临床和流行病学数据。通过其形态和生理特征对分离株进行鉴定。采用T3B聚合酶链反应（PCR）指纹图谱技术对物种进行分子鉴定。
结果
在表型试验中，34株分离株被鉴定为巴西孢子丝菌，1株为申克孢子丝菌, 12株为孢子丝菌属。PCR鉴定所有分离株均为巴西孢子丝菌。
主要结论
迄今为止，巴西孢子丝菌是里约热内卢猫孢子丝菌病的唯一病原体。未发现分离株与临床和流行病学数据之间存在关联。此外，我们强烈建议使用分子技术来鉴定孢子丝菌属的分离株。
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