α-Ketoglutarate stimulates pendrin-dependent Cl absorption in the mouse CCD through protein kinase C.

α-Ketoglutarate (α-KG) is a citric acid cycle intermediate and a glutamine catabolism product. It is also the natural ligand of 2-oxoglutarate receptor 1 (OXGR1), a G protein-coupled receptor expressed on the apical membrane of intercalated cells. In the cortical collecting duct (CCD), Cl/[Formula: see text] exchange increases upon α-KG binding to the OXGR1. To determine the signaling pathway(s) by which α-KG stimulates Cl absorption, we examined α-KG-stimulated Cl absorption in isolated perfused mouse CCDs. α-KG increased electroneutral Cl absorption in CCDs from wild-type mice but had no effect on Cl absorption in pendrin knockout mice. Because G protein-coupled receptors activate PKC, we hypothesized that α-KG stimulates Cl absorption through PKC. If so, PKC agonists should mimic, whereas PKC inhibitors should abolish, α-KG-stimulated Cl absorption. Like α-KG, PKC agonist (phorbol-12,13-dibutyrate, 500 nM) application increased Cl absorption in wild-type but not in pendrin null CCDs. Moreover, PKC inhibitors (2.5 mM GF109203X and 20 nM calphostin C), Ca chelators (BAPTA, 10-20 μM), or PKC-α or -δ gene ablation eliminated α-KG-stimulated Cl absorption. We have shown that STE20/SPS-1-related proline-alanine-rich protein kinase (SPAK) gene ablation increases urinary α-KG excretion, renal pendrin abundance, and CCD Cl absorption. However, in SPAK null CCDs, Cl absorption was not activated further by luminal α-KG application nor was Cl absorption reduced with the PKC inhibitor GF109203 . Thus SPAK gene ablation likely acts through a PKC-independent pathway to produce a chronic adaptive increase in pendrin function. In conclusion, α-KG stimulates pendrin-dependent Cl/[Formula: see text] exchange through a mechanism dependent on PKC and Ca that involves PKC-α and PKC-δ.