Department of Pharmacognosy, Wrocław Medical University, ul. Borowska 211a, 50-556 Wrocław, Poland.
Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, R. Boškovića 35, 21000 Split, Croatia.
J Pharm Biomed Anal. 2018 Apr 15;152:128-136. doi: 10.1016/j.jpba.2018.01.046. Epub 2018 Feb 3.
The scope of current study was to apply supercritical CO for extraction of black poplar buds (Populus nigra L.) and to optimize the extraction parameters (pressure and temperature) using response surface methodology aimed at maximum extraction yield, total phenolic content (TP), ferric reducing antioxidant power (FRAP) and individual amount of selected bioactive phenolics. The pressure and temperature influenced significantly the extract yield (0.38-10.28 g/100 g), TP content (21.62-31.09 μg GAE/mg), FRAP capacity (0.30-0.48 μmol Fe/mg), and the amounts of p-coumaric acid (0.33-1.52 μg/mg), pinocembrin (20.99-47.24 μg/mg) and galangin (4.04-10.25 μg/mg). The extraction of pinostrobin (51.73-79.56 μg/mg), pinobanksin (0.41-1.55 μg/mg), and chrysin (0.97-2.03 μg/mg) was significantly affected only by the operating pressure. Optimized extraction parameters (30 MPa and 60 °C) allow to obtain higher yield and extracts enriched with bioactive phenolics.
本研究旨在应用超临界 CO2 萃取黑杨芽（Populus nigra L.），并通过响应面法优化萃取参数（压力和温度），以获得最大萃取收率、总酚含量（TP）、铁还原抗氧化能力（FRAP）和选定生物活性酚类物质的含量。压力和温度显著影响萃取收率（0.38-10.28 g/100 g）、TP 含量（21.62-31.09 μg GAE/mg）、FRAP 能力（0.30-0.48 μmol Fe/mg）以及对香豆酸（0.33-1.52 μg/mg）、松属素（20.99-47.24 μg/mg）和乔松素（4.04-10.25 μg/mg）的含量。松柏醇（51.73-79.56 μg/mg）、松脂素（0.41-1.55 μg/mg）和白杨素（0.97-2.03 μg/mg）的萃取仅受操作压力显著影响。优化的萃取参数（30 MPa 和 60 °C）可获得更高的收率和富含生物活性酚类物质的提取物。
