带有双乙酰氧基部分的探针用于检测肼及其在活细胞中的应用。
A probe with double acetoxyl moieties for hydrazine and its application in living cells.
机构信息
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5;203:106-111. doi: 10.1016/j.saa.2018.05.112. Epub 2018 May 29.
As a common chemical reductant, hydrazine has been widely used in various fields. However, its high toxicity to human and environment have also attracted people's attention. In this work, a new fluorescence "turn-on" probe based on coumarin for hydrazine was successfully synthesized. The probe with double acetoxyl moieties as the reaction sites can obtain the detection limit as low as 2.98 nM for the detection of hydrazine in distilled water, which was lower than the U.S. Environmental Protection Agency standard (10 ppb). In addition, it also responded obvious fluorescence enhancement and high selectivity to hydrazine over other molecules. Furthermore, this probe could visualize the hydrazine in living cells.
作为一种常见的化学还原剂，肼在各个领域得到了广泛的应用。然而，它对人类和环境的高毒性也引起了人们的关注。在这项工作中，成功合成了一种基于香豆素的新的荧光“开启”探针用于检测肼。该探针具有双乙酰氧基作为反应位点，在蒸馏水中检测肼的检测限低至 2.98 nM，低于美国环境保护署的标准（10 ppb）。此外，它对肼的响应也表现出明显的荧光增强和对其他分子的高选择性。此外，该探针还可以在活细胞中可视化肼。
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