一种新型基于荧光素的“开启”探针，用于检测联氨及其在活细胞中的应用。
A novel fluorescein-based "turn-on" probe for the detection of hydrazine and its application in living cells.
机构信息
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China.
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Mar 15;193:324-329. doi: 10.1016/j.saa.2017.12.040. Epub 2017 Dec 14.
We constructed a novel probe for hydrazine detection based on ICT and PET mechanism. Phthalimide and acetyl ester groups were used as the recognition units. Addition of hydrazine produced a turn-on fluorescence at 525nm along with the fluorescent color change from dark to yellow. The probe could selectively detect hydrazine over other related interfering species. The detection limit of the probe for hydrazine was calculated to be 0.057μM which was lower than the EPA standard (0.320μM). Furthermore, the probe could also be applied for the imaging of hydrazine in living cells.
我们基于 ICT 和 PET 机制构建了一种用于检测联氨的新型探针。邻苯二甲酰亚胺和乙酰酯基被用作识别单元。联氨的加入在 525nm 处产生了一个开式荧光，同时荧光颜色从暗到黄发生变化。该探针可以选择性地检测联氨，而其他相关干扰物质则不会。该探针对联氨的检测限计算为 0.057μM，低于 EPA 标准（0.320μM）。此外，该探针还可用于活细胞中联氨的成像。
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