Ando Kaori, Rynearson Sheri, Muleta Kebede T, Gedamu Jhonatan, Girma Bedada, Bosque-Pérez Nilsa A, Chen Ming-Shun, Pumphrey Mike O
Department of Crop and Soil Sciences, Washington State University, Pullman, Washington, United States of America.
Ethiopian Institute of Agricultural Research, Holeta Agricultural Research Center, Holeta, Ethiopia.
PLoS One. 2018 Feb 7;13(2):e0191305. doi: 10.1371/journal.pone.0191305. eCollection 2018.
Northern areas of the western United States are one of the most productive wheat growing regions in the United States. Increasing productivity through breeding is hindered by several biotic stresses which slow and constrain targeted yield improvement. In order to understand genetic variation for stripe rust (Puccinia striiformis f. sp. tritici), Septoria tritici blotch (Mycosphaerella graminicola), and Hessian fly (Mayetiola destructor) in regional germplasm, a panel of 408 elite spring wheat lines was characterized and genotyped with an Illumina 9K wheat single nucleotide polymorphism (SNP) chip to enable genome-wide association study (GWAS) analyses. Significant marker-trait associations were identified for stripe rust (38 loci), Septoria tritici blotch (8) and Hessian fly (9) resistance. Many of the QTL corresponded with previously reported gene locations or QTL, but we also discovered new resistance loci for each trait. We validated one of the stripe rust resistance loci detected by GWAS in a bi-parental mapping population, which confirmed the detection of Yr15 in the panel. This study elucidated well-defined chromosome regions for multiple pest resistances in elite Northwest germplasm. Newly identified resistance loci, along with SNPs more tightly linked to previously reported genes or QTL will help future breeding and marker assisted selection efforts.
美国西部的北部地区是美国小麦种植最丰产的地区之一。通过育种提高产量受到多种生物胁迫的阻碍，这些胁迫减缓并限制了目标产量的提高。为了了解区域种质中条锈病（条形柄锈菌小麦专化型）、小麦叶枯病（小麦壳针孢）和黑森瘿蚊（麦瘿蚊）的遗传变异，对408个优良春小麦品系组成的群体进行了特征鉴定，并使用Illumina 9K小麦单核苷酸多态性（SNP）芯片进行基因分型，以开展全基因组关联研究（GWAS）分析。鉴定出了与条锈病抗性（38个位点）、小麦叶枯病抗性（8个）和黑森瘿蚊抗性（9个）相关的显著标记-性状关联。许多数量性状位点（QTL）与先前报道的基因位置或QTL一致，但我们也发现了每个性状的新抗性位点。我们在一个双亲作图群体中验证了GWAS检测到的一个条锈病抗性位点，这证实了该群体中Yr15的检测。本研究阐明了西北优良种质中多个害虫抗性的明确染色体区域。新鉴定的抗性位点，以及与先前报道的基因或QTL更紧密连锁的SNP，将有助于未来的育种和标记辅助选择工作。
