DE Colli Marianna, Radunovic Milena, Zizzari Vincenzo L, DI Giacomo Viviana, DI Nisio Chiara, Piattelli Adriano, Calvo Guirado José L, Zavan Barbara, Cataldi Amelia, Zara Susi
Department of Pharmacy, University "G. d'Annunzio" Chieti-Pescara.
School of Dental Medicine, University of Belgrade.
Dent Mater J. 2018 Mar 30;37(2):197-205. doi: 10.4012/dmj.2016-418. Epub 2018 Feb 8.
Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.
钛表面改性对牙种植体的成功至关重要。我们的目的是确定表面对牙髓干细胞（DPSC）活力和分化的影响。将种植体分为喷砂/酸蚀（对照）组和涂覆有钙和镁离子（CaMg）的喷砂/酸蚀组（作为复合材料供应，为试验组）。通过MTT评估增殖，通过检测成骨细胞基因表达、PGE2分泌和基质形成来检查分化，通过检测白细胞介素6（IL-6）来评估炎症。试验组的MTT和IL-6没有变化。记录到试验组的PGE2增加。在早期实验点，试验组的BMP2较高，后期Osterix和RUNX2增加。茜素红S显示试验组的基质产生更高。这些结果表明，试验表面具有更强的骨诱导性，这为旨在构建具有更高生物相容性的牙种植体的体内研究提供了一个起点，这种牙种植体的整合和临床性能得到改善，一些不良影响，如种植体稳定性丧失和进一步的外科手术，也会减少。
