Di Carlo Roberta, Zara Susi, Ventrella Alessia, Siani Gabriella, Da Ros Tatiana, Iezzi Giovanna, Cataldi Amelia, Fontana Antonella
Department of Pharmacy, University "G. d'Annunzio", Via dei Vestini, 66100 Chieti, Italy.
Department of Chemical and Pharmaceutical Sciences, University of Trieste, Piazzale Europa 1, 34127 Trieste, Italy.
Nanomaterials (Basel). 2019 Apr 12;9(4):604. doi: 10.3390/nano9040604.
(1) Background: The aim of this study was to optimize, through a cheap and facile protocol, the covalent functionalization of graphene oxide (GO)-decorated cortical membrane (Lamina) in order to promote the adhesion, the growth and the osteogenic differentiation of DPSCs (Dental Pulp Stem Cells); (2) Methods: GO-coated Laminas were fully characterized by Scannsion Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) analyses. In vitro analyses of viability, membrane integrity and calcium phosphate deposition were performed; (3) Results: The GO-decorated Laminas demonstrated an increase in the roughness of Laminas, a reduction in toxicity and did not affect membrane integrity of DPSCs; and (4) Conclusions: The GO covalent functionalization of Laminas was effective and relatively easy to obtain. The homogeneous GO coating obtained favored the proliferation rate of DPSCs and the deposition of calcium phosphate.
(1) 背景：本研究的目的是通过一种廉价且简便的方案，优化氧化石墨烯（GO）修饰的皮质膜（板层）的共价功能化，以促进牙髓干细胞（DPSCs）的黏附、生长和成骨分化；(2) 方法：通过扫描电子显微镜（SEM）和原子力显微镜（AFM）分析对GO包被的板层进行全面表征。进行了活力、膜完整性和磷酸钙沉积的体外分析；(3) 结果：GO修饰的板层显示出板层粗糙度增加、毒性降低且不影响DPSCs的膜完整性；(4) 结论：板层的GO共价功能化是有效的且相对容易实现。获得的均匀GO涂层有利于DPSCs的增殖率和磷酸钙的沉积。
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