原代人成骨细胞在微粗糙钛表面的体外行为

In Vitro Behavior of Primary Human Osteoblasts Onto Microrough Titanium Surface.

作者信息

Zizzari Vincenzo Luca, Marconi Guya Diletta, De Colli Marianna, Zara Susi, Zavan Barbara, Salini Vincenzo, Fontana Antonella, Cataldi Amelia, Piattelli Adriano

机构信息

*Research Fellow, Department of Pharmacy, University of "G. d'Annunzio," Chieti, Italy. †PhD Student, Department of Pharmacy, University of "G. d'Annunzio," Chieti, Italy. ‡Research Fellow, Department of Pharmacy, University of "G. d'Annunzio," Chieti, Italy. §Researcher, Department of Pharmacy, University of "G. d'Annunzio," Chieti, Italy. ||Researcher, Department of Biomedical Sciences, University of Padova, Padova, Italy. ¶Professor, Department of Medicine and Aging Sciences, University of "G. d'Annunzio," Chieti, Italy. #Professor, Department of Pharmacy, University of "G. d'Annunzio," Chieti, Italy. **Professor, Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio," Chieti, Italy.

出版信息

Implant Dent. 2015 Aug;24(4):377-83. doi: 10.1097/ID.0000000000000268.

DOI:10.1097/ID.0000000000000268

PMID:25915409

Abstract

OBJECTIVE

The aim of this study was to evaluate in vitro the behavior and the biocompatibility of primary human osteoblasts (HOs) grown onto different implant surface.

METHODS AND MATERIALS

HOs were cultured onto sandblasted/acid-etched (control group) and sandblasted/acid-etched followed by coating with inorganic ions (test group) experimental titanium discs. At established times, SEM analysis, LDH assay, MTT assay, and enzyme-linked immunosorbent assay for type 1 collagen, interleukin (IL)-6, and PGE2 secretion were performed.

RESULTS

Both surfaces promote HOs adhesion and proliferation. After 21 days, cells on test surfaces are well spread, flattened, and attached by cellular extensions, whereas cells on control discs appear mainly elongated. Lower LDH levels and higher values of MTT assay are recorded for cells on test respect to control surfaces at each experimental time. Type 1 collagen release increases until 14 days, significantly decreasing at day 21 in cells grown on both surfaces. IL-6 and PGE2 secretion shows a peak in control group samples at day 7, whereas their levels do not significantly modify in both groups at days 14 and 21.

CONCLUSION

Results indicate that the test group surface is more biocompatible, well tolerated, and suitable for supporting osteoblasts growth and proliferation.

摘要

目的

本研究旨在体外评估原代人成骨细胞（HOs）在不同种植体表面的行为和生物相容性。

方法和材料

将HOs培养在喷砂/酸蚀（对照组）以及喷砂/酸蚀后再涂覆无机离子（试验组）的实验性钛盘上。在既定时间进行扫描电子显微镜（SEM）分析、乳酸脱氢酶（LDH）测定、噻唑蓝（MTT）测定以及针对I型胶原蛋白、白细胞介素（IL）-6和前列腺素E2（PGE2）分泌的酶联免疫吸附测定。

结果

两种表面均促进HOs的黏附和增殖。21天后，试验表面上的细胞铺展良好、扁平，并通过细胞突起附着，而对照盘上的细胞主要呈细长形。在每个实验时间点，试验表面上的细胞相对于对照表面记录到较低的LDH水平和较高的MTT测定值。I型胶原蛋白释放量在14天前增加，在两种表面上生长的细胞中于第21天显著下降。IL-6和PGE2分泌在对照组样本中于第7天出现峰值，而在第14天和第21天两组中的水平均无显著变化。