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噬菌体鸡尾酒包含 Podoviridae 和 Myoviridae 噬菌体，可抑制体外和体内条件下的果胶杆菌生长。

Phage cocktail containing Podoviridae and Myoviridae bacteriophages inhibits the growth of Pectobacterium spp. under in vitro and in vivo conditions.

机构信息

Institute for Global Food Security, School of Biological Sciences, Queen's University, Belfast, Northern Ireland, United Kingdom.

Sustainable Agri-Food Sciences Division, Agri-Food and Biosciences Institute, Belfast, Northern Ireland, United Kingdom.

出版信息

PLoS One. 2020 Apr 2;15(4):e0230842. doi: 10.1371/journal.pone.0230842. eCollection 2020.

DOI:10.1371/journal.pone.0230842
PMID:32240203
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7117878/
Abstract

Globally, there is a high economic burden caused by pre- and post-harvest losses in vegetables, fruits and ornamentals due to soft rot diseases. At present, the control methods for these diseases are limited, but there is some promise in developing biological control products for use in Integrated Pest Management. This study sought to formulate a phage cocktail which would be effective against soft rot Pectobacteriaceae species affecting potato (Solanum tuberosum L.), with potential methods of application in agricultural systems, including vacuum-infiltration and soil drench, also tested. Six bacteriophages were isolated and characterized using transmission electron microscopy, and tested against a range of Pectobacterium species that cause soft rot/blackleg of potato. Isolated bacteriophages of the family Podoviridae and Myoviridae were able to control isolates of the Pectobacterium species: Pectobacterium atrosepticum and Pectobacterium carotovorum subsp. carotovorum. Genomic analysis of three Podoviridae phages did not indicate host genes transcripts or proteins encoding toxin or antibiotic resistance genes. These bacteriophages were formulated as a phage cocktail and further experiments showed high activity in vitro and in vivo to suppress Pectobacterium growth, potentially indicating their efficacy in formulation as a microbial pest control agent to use in planta.

摘要

全球范围内，由于软腐病，蔬菜、水果和观赏植物在收获前后都会造成严重的经济损失。目前，这些疾病的控制方法有限，但在开发用于病虫害综合治理的生物防治产品方面有一些希望。本研究旨在配制一种噬菌体鸡尾酒，以有效防治影响马铃薯（Solanum tuberosum L.）的软腐果胶杆菌属物种，同时还测试了在农业系统中应用的潜在方法，包括真空渗透和土壤淋洗。使用透射电子显微镜分离和表征了六种噬菌体，并对引起马铃薯软腐/黑胫的一系列果胶杆菌属物种进行了测试。分离出的科 Podoviridae 和 Myoviridae 噬菌体能够控制果胶杆菌属的分离株：果胶杆菌属软腐亚种和果胶杆菌属胡萝卜软腐亚种。对三个 Podoviridae 噬菌体的基因组分析表明，没有宿主基因转录本或编码毒素或抗生素抗性基因的蛋白质。这些噬菌体被配制成噬菌体鸡尾酒，进一步的实验表明，它们在体外和体内都具有很高的活性，能够抑制果胶杆菌的生长，这可能表明它们在作为植物内生微生物防治剂的配方中具有功效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/ba227e36742b/pone.0230842.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/17a95de01303/pone.0230842.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/bf170de64a78/pone.0230842.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/cd7d6d6be4ac/pone.0230842.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/6d47674d2e83/pone.0230842.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/28d402ed0436/pone.0230842.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/ba227e36742b/pone.0230842.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/17a95de01303/pone.0230842.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/bf170de64a78/pone.0230842.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/cd7d6d6be4ac/pone.0230842.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/6d47674d2e83/pone.0230842.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/28d402ed0436/pone.0230842.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c79/7117878/ba227e36742b/pone.0230842.g006.jpg

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