Faculty of Medicine, Institute of Medical Physiology "Richard Burian", University of Belgrade, Belgrade, Serbia.
Department of Chemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia.
Mol Cell Biochem. 2018 Nov;448(1-2):43-50. doi: 10.1007/s11010-018-3311-2. Epub 2018 Feb 8.
The aim of this study was to assess the effects of L-cysteine (Cys) (7 mg/kg) and N-acetyl-L-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.
本研究旨在评估 L-半胱氨酸 (Cys) (7mg/kg) 和 N-乙酰-L-半胱氨酸 (NAC) (50mg/kg) 在亚慢性腹腔注射蛋氨酸 (Met) (0.8mmol/kg) 21 天后对大鼠肝脏的影响。在肝组织中测定丙二醛 (MDA) 浓度、谷胱甘肽含量 (GSH)、过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GPx) 和乙酰胆碱酯酶 (AchE) 活性，并测定血浆/血清中肝酶 (AST、ALT、ALP 和 GGT) 的活性和总蛋白和白蛋白的浓度。Cys 和 NAC 增加了 CAT、SOD 和 AchE 的活性。Met 导致门脉周围单核细胞浸润和罕见的局灶性肝细胞坏死。在补充 Cys 和 NAC 的组中，观察到细胞内水肿和微泡脂肪变性而无坏死。我们观察到在蛋氨酸处理组中 AST、ALT 和 ALP 活性降低。我们的结果表明，Cys 和 NAC 的应用可以增加抗氧化酶的活性，并在亚慢性蛋氨酸暴露的情况下防止肝脏发生强烈的组织学变化。
