Rakhmanin Yu A, Ivanova L V, Artemova T Z, Gipp E K, Zagaynova A V, Maksimkina T N, Krasnyak A V, Zhuravlev P V, Aleshnya V V, Panasovets O P
Gig Sanit. 2016;95(6):582-8.
The used methodology of the scientific substantiation of indicators is in the establishment of the conformity of laws of vital activity of indicator and pathogenic microorganisms in the real conditions of the action of the complex of factors, including disinfecting agents. In the one water sample simultaneously there were determined both the general indicator (GICB), thermotolerant (TTCB), glucose positive (GPCB) coliform bacteria, E.coli. On the base of long-term research in the various regions of the Russian Federation, as well with bearing in mind the analysis of domestic and foreign data, comparing the water quality and the incidence of intestinal infections in population it is recommended to use the index of determination of the total number glucose positive coliform bacteria (GPCB), which brings together a much broader range of bacteria of the Enterobacteriaceae family in comparison with total coliform bacteria (TCB) and thermotolerant coliform bacteria (TTCB) and warrants the absence in the test volume of water as an indicator lactose positive (E.coli, TCB, TTCB) and pathogens (salmonella) and potentially pathogenic bacteria which do not ferment lactose. Proposed index of GPCB is shown to allow to assess epidemiological risks not only more accurate, but also more efficiently without increasing the cost performance of bacteriological research.
用于指标科学论证的方法是在包括消毒剂在内的多种因素作用的实际条件下,确定指标的生命活动规律与致病微生物的一致性。在一个水样中同时测定了总指示菌(GICB)、耐热性指示菌(TTCB)、葡萄糖阳性指示菌(GPCB)、大肠埃希菌。基于在俄罗斯联邦不同地区的长期研究,并考虑到对国内外数据的分析,比较水质与人群肠道感染发病率,建议使用葡萄糖阳性总大肠菌群数(GPCB)的测定指标,与总大肠菌群(TCB)和耐热性大肠菌群(TTCB)相比,该指标涵盖了更广泛的肠杆菌科细菌,并且保证在测试水样中不存在作为乳糖阳性指示菌(大肠埃希菌、TCB、TTCB)和病原体(沙门氏菌)以及不发酵乳糖的潜在致病菌。结果表明,所提出的GPCB指标不仅能更准确地评估流行病学风险,而且能更有效地评估,同时不会增加细菌学研究的成本效益。