Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Clin Microbiol Infect. 2011 Feb;17(2):223-31. doi: 10.1111/j.1469-0691.2010.03283.x.
Hepatitis B virus (HBV) is classified into eight major genotypes, A-H, which are geographically distributed worldwide. The aim of this work was to describe the clinical characteristics associated with the HBV genotypes circulating in Buenos Aires city. The study included 139 patients infected with HBV, whose clinical courses were classified as acute symptomatic self-limiting hepatitis, inactive carrier state and chronic active hepatitis (HBV e-antigen (HBeAg)-positive and HBeAg-negative). The HBV genotypes were determined in 128 patients by PCR-restriction fragment length polymorphism and phylogenetic analysis. Biochemical, virological, clinical and histological features were analysed. A differential distribution of genotypes between acute symptomatic and chronic infections was found. Among the acute cases, genotype F was predominant (65.2%, 30/46) and genotype D was rare (4.3%, 2/46), whereas among the chronic infections, a homogeneous distribution of genotypes A (26.8%, 22/82), D (31.7%, 26/82) and F (36.6%, 30/82), with an unusual presence of genotypes B (1.2%, 1/82) and C (3.7%, 3/82), was observed. Regarding the liver histology of chronically infected patients, genotype F tended to display higher histological activity indexes. Mutations related to HBV surface antigen immunoreactivity, antiviral resistance and HBeAg-negative status were studied. This work constitutes, to our knowledge, the first description of the clinical characteristics related to HBV genotypes in Argentina, where the distribution of genotypes in patients with acute infection has not been reported previously. Finally, it was established that genotype F is the prevalent genotype among the acute symptomatic infections in Buenos Aires city, and that it shows a tendency to cause an adverse disease outcome among the chronic cases.
乙型肝炎病毒（HBV）分为 A-H 八个主要基因型，在全球范围内具有地理分布。本研究的目的是描述在布宜诺斯艾利斯市流行的 HBV 基因型相关的临床特征。该研究纳入了 139 例 HBV 感染者，其临床病程分为急性自限性症状性肝炎、非活动性携带者状态和慢性活动性肝炎（HBV e 抗原（HBeAg）阳性和 HBeAg 阴性）。通过聚合酶链反应-限制性片段长度多态性和系统进化分析确定了 128 例患者的 HBV 基因型。分析了生化、病毒学、临床和组织学特征。发现基因型在急性症状性和慢性感染之间存在差异分布。在急性病例中，基因型 F 占主导地位（65.2%，30/46），而基因型 D 则很少见（4.3%，2/46），而在慢性感染中，基因型 A（26.8%，22/82）、D（31.7%，26/82）和 F（36.6%，30/82）的分布均匀，而基因型 B（1.2%，1/82）和 C（3.7%，3/82）的存在则不同。关于慢性感染患者的肝组织学，基因型 F 倾向于显示更高的组织学活动指数。研究了与 HBV 表面抗原免疫反应性、抗病毒耐药性和 HBeAg 阴性状态相关的突变。据我们所知，这是阿根廷首次描述与 HBV 基因型相关的临床特征的研究，此前尚未报道过急性感染患者基因型的分布。最后，确定基因型 F 是布宜诺斯艾利斯市急性症状性感染的主要流行基因型，并且在慢性病例中表现出不良疾病结局的趋势。
