Cintra Maria Christine Rizzon, Ollhoff Rüdiger Daniel, Sotomaior Cristina Santos
Graduate Program in Animal Science, School of Life Sciences-Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Paraná 80215-901, Brazil.
Graduate Program in Animal Science, School of Life Sciences-Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Paraná 80215-901, Brazil.
Vet Parasitol. 2018 Feb 15;251:106-111. doi: 10.1016/j.vetpar.2018.01.005. Epub 2018 Jan 5.
The FAMACHA© system is used for targeted selective treatment (TST) of small ruminants for the control of haemonchosis. The aim of this study was to evaluate the sensitivity and specificity of this system in growing lambs. Between 2015 and 2016, lambs aged 60-210 days on two sheep farms were evaluated at 15-day intervals using the FAMACHA© system (F1-F5), with analysis of hematocrit (Ht), counting of eggs per gram of feces, and larval culture. The sensitivity and specificity of the system were calculated according to two criteria: criterion 1, lambs classified as F4 or F5 were considered anemic (positive test) and those classified as F1, F2, or F3 were considered not anemic (negative test); and criterion 2, lambs classified as F3, F4, or F5 were considered anemic (positive test) and those classified as F1 and F2 were considered not anemic (negative test). Three gold standard Ht cutoff values were used to diagnose anemia, i.e., ≤22%, ≤18%, and ≤15%. In total, 1591 assessments were analyzed. The highest percentage of larvae in the fecal cultures was for Haemonchus spp., with an average of 78.5%. The sensitivity values found when F3 lambs were considered anemic were 13.9%, 30.8%, and 66.7%, respectively, for Ht cutoff values of ≤22%, ≤18%, and ≤15%. When F3 lambs were excluded, the maximum sensitivity was 14.9% and the specificity was 100% for all degrees of anemia using criterion 1. The FAMACHA© system had low sensitivity in growing lambs and should not be used alone for control of haemonchosis in young animals.
FAMACHA©系统用于对小型反刍动物进行针对性的选择性治疗（TST）以控制血矛线虫病。本研究的目的是评估该系统在生长羔羊中的敏感性和特异性。在2015年至2016年期间，对两个养羊场中60 - 210日龄的羔羊每隔15天使用FAMACHA©系统（F1 - F5）进行评估，并分析血细胞比容（Ht）、每克粪便中的虫卵计数以及幼虫培养情况。该系统的敏感性和特异性根据两个标准计算：标准1，分类为F4或F5的羔羊被视为贫血（阳性检测），分类为F1、F2或F3的羔羊被视为非贫血（阴性检测）；标准2，分类为F3、F4或F5的羔羊被视为贫血（阳性检测），分类为F1和F2的羔羊被视为非贫血（阴性检测）。使用三个金标准Ht临界值来诊断贫血，即≤22%、≤18%和≤15%。总共分析了1591次评估。粪便培养物中幼虫比例最高的是血矛线虫属，平均为78.5%。当F3羔羊被视为贫血时，对于Ht临界值≤22%、≤18%和≤15%，发现的敏感性值分别为13.9%、30.8%和66.7%。当排除F3羔羊时，使用标准1，对于所有程度的贫血，最大敏感性为14.9%，特异性为100%。FAMACHA©系统在生长羔羊中的敏感性较低，不应单独用于控制幼龄动物的血矛线虫病。
